微管相关蛋白样激酶1在胃癌组织中的表达及其临床意义  

Expression and clinical pathological significance of doublecortin-like kinase 1 in gastric cancer tissue

作　　者：贾长河[1] 谢毅[2] 袁媛[1] 刘博伟[1] 任颖[3] JIA Changhe;XIE Yi;YUAN Yuan;LIU Bowei;REN Ying(Department of Gastroenterology,Henan Provincial People’s Hospital,Zhengzhou,Henan 450003,China;Department of Gastrointestinal Surgery,Henan Provincial People’s Hospital,Zhengzhou,Henan 450003,China;Department of Pathology,Henan Provincial People’s Hospital,Zhengzhou,Henan 450003,China)

机构地区：[1]河南省人民医院消化科,河南郑州450003 [2]河南省人民医院胃肠外科,河南郑州450003 [3]河南省人民医院病理科,河南郑州450003

出　　处：《安徽医药》2020年第2期271-275,I0002,共6页Anhui Medical and Pharmaceutical Journal

基　　金：河南省科学技术厅科技发展计划项目(162102310275)

摘　　要：目的研究微管相关蛋白样激酶1(doublecortin-like kinase 1,DCLK1)在胃癌组织中的表达及其临床意义。方法回顾性分析2006年5月至2011年7月在河南省人民医院住院治疗的267例胃癌病人临床病理资料,免疫组化染色法检测267例胃癌组织和138例癌旁正常组织中DCLK1的表达,分析DCLK1表达与胃癌病人临床病理特征之间的关系,Cox风险回归模型回归分析影响胃癌病人预后生存的影响因素,Kaplan-Meier法绘制不同DCLK1表达水平病人的5年总体生存和无复发生存曲线,Log-rank法比较5年总体生存和无复发生存的差异。结果 DCLK1在胃癌组织中的高表达率71.9%(192/267)高于癌旁正常组织26.1%(36/138)。单因素分析显示DCLK1高表达与病人性别、年龄,肿瘤大小,位置等临床病理因素之间无显著关联(P>0.05),与肿瘤分化程度、浸润深度、神经侵犯、脉管侵犯、淋巴转移及临床分期等临床病理因素之间有关(P<0.05)。Cox回归分析显示DCLK1高表达、临床分期和肿瘤分化程度是胃癌病人总生存率和无复发生存率的独立影响因素(P<0.05)。Kaplan-Meier生存分析显示,DCLK1高表达的胃癌病人的总生存率和无复发生存率较低(P<0.05)。结论 DCLK1在胃癌组织中高表达是胃癌病人预后生存的独立影响因素。Objective To investigate the expression and clinical pathological significance of doublecortin-like kinase 1(DCLK1)in gastric cancer tissues.Methods Clinical pathological data of 267 patients with gastric cancers hospitalized from May 2006 to July 2011 were retrospectively reviewed.Expression of DCLK1 in gastric cancer tissues and adjacent normal tissues from 267 cases and 138 cases respectively was determined by immnunohistochemistry.The relationship between DCLK1 expression and clinical pahtological parameters was analyzed.Cox analysis was performed to analyze the independent prognostic factors of patients with gastric cancer.The 5-year overall survival and recurrence-free survival curves of patients with different DCLK1 expression levels were drawn by Kaplan-Meier method and the 5-year overall survival and recurrence-free rates were compared by Log-rank method.Results The high expression rate of DCLK1 in gastric cancer tissues was 71.9%(192/267),which was higher than that in adjacent normal tissues 26.1%(36/138).High expression of DCLK1 in gastric cancer tissues did not correlate with gender,age,tumor size and site(P>0.05). High expression of DCLK1 statistically significantly correlated with tumor differentiation,depth of invasion,nerve infiltration,venous infiltration,lymph nodal metastasis and clinical stage(P<0.05).Cox analysis indicated that DCLK1 high expression,clinical stage and tumor differentiation were independent factors in the overall survival rate and recurrence-free survival rate of gastric cancer patients(P<0.05).Kaplan-Meier survival analysis showed that the overall survival rate and recurrence-free survival rate of patients with gastric cancer with high DCLK1 expression were lower(P<0.05).Conclusion DCLK1 high expression is an independent prognostic factor of gastric cancer patients.

关 键 词：胃肿瘤 微管相关蛋白质类 免疫组织化学 预后 转移 

分 类 号：R735.2[医药卫生—肿瘤]