不同剂量γ射线照射对肺癌细胞分化、增殖、凋亡以及放疗敏感性的影响  

Experimental study on the effects of different doses of Gamma ray irradiation ondifferentiation,proliferation,apoptosis and radiotherapy sensitivity of lung cancer cells

作　　者：饶石磊[1] 杨峥[1] 王旸[1] 齐书然 张凯 RAO Shilei;YANG Zheng;WANG Yang;QI Shuran;ZHANG Kai(Department of Radiotherapy,Nanyang Central Hospital,Nanyang,Henan 473000,China)

机构地区：[1]南阳市中心医院放疗科

出　　处：《安徽医药》2020年第2期281-285,I0003,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨不同剂量γ射线照射对肺癌细胞分化、增殖、凋亡以及放疗敏感性的影响。方法体外培养的人肺癌细胞株A549采用随机数字表法分为对照组、2戈瑞(Gy)组、4 Gy组、6 Gy组、8 Gy组、10 Gy组,以不同剂量的γ射线照射细胞,以细胞克隆形成实验检测细胞存活情况;以CKK-8法检测细胞增殖情况,计算细胞的生长抑制率,检测各组的放疗敏感性;以流式细胞技术检测细胞凋亡情况;采用免疫印记(WB)法检测肺组织分化标志物粘蛋白MUC-1,肺泡Ⅱ型上皮细胞特异性蛋白标志物SPC1、SPC2的表达;以Transwell细胞体外侵袭实验检测细胞的侵袭能力。结果与对照组比较,照射组细胞相对细胞克隆形成、MUC-1蛋白表达、侵袭能力降低,生长抑制率、凋亡率、SPC1、SPC2蛋白表达增高且呈剂量依赖性(P<0.05);照射组细胞的放疗敏感性呈下降趋势,且6 Gy组、8 Gy组、10 Gy组与2 Gy组相比有统计学意义(P<0.05)。结论γ射线照射可抑制人肺癌细胞株A549增殖、促进其凋亡、促进其分化、减轻其恶性程度且随剂量增加效果增强;但其放疗敏感性随剂量增加呈下降趋势。Objective To study the effects of different doses of radiation on differentiation,proliferation,apoptosis and radiosensitivity of lung cancer cells.Methods Human lung cancer cell line A549 in vitro was randomly divided into control group,2 Gy group,4 Gy group,6 Gy group,8 Gy group and 10 Gy group.Cells were irradiated with different doses of gamma rays,cell viability was measured by cell clone formation assay,CKK-8 method was used to detect cell proliferation,cell growth inhibition rate was calculated,theradiosensitivity of each group was detected,cell apoptosis was detected by flow cytometry,the expressions of mucin MUC-1,a marker of lung tissue differentiation,SPC1 and SPC2,the specific protein markers of alveolar type II epithelial cells,were detected by immunoblotting(WB),and the invasive ability of Transwell cells was tested by in vitro invasion test.Results Compared with control group,the relative cell clone formation,MUC-1 protein expression and invasive ability of cells in irradiation group decreased,and the growth inhibition rate,apoptosis rate,SPC1 and SPC2 protein expressions increased in a dose-dependent manner(P<0.05).The radiosensitivity of cells in irradiation group decreased,and there was statistical significance between 6 Gy group,8 Gy group,10 Gy group and 2 Gy group(P<0.05).Conclusion Gamma irradiation can inhibit the proliferation of human lung cancer cell line A549,promote its apoptosis,promote its differentiation,alleviate its malignant degree and enhance its effect with the increase of dose,but its radiosensitivity decreases with the increase of dose.

关 键 词：肺肿瘤 Γ射线 放射治疗剂量 黏蛋白类 肺泡表面活性蛋白C 增殖 凋亡 分化 

分 类 号：R734.2[医药卫生—肿瘤]