外源L-天冬氨酸纳米钙抑制棉花生长的效应及潜在机制  

Effects of exogenous calcium L-aspartate nanoparticles on cotton growth and its potential mechanisms

作　　者：刘亚林 闫磊[1] 曾钰 贾志贤 姜存仓[1] LIU Ya-lin;YAN Lei;ZENG Yu;JIA Zhi-xian;JIANG Cun-cang(Microelement Research Center,Huazhong Agricultural University,College of Resources and Environment,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学微量元素研究中心资源与环境学院

出　　处：《农业环境科学学报》2020年第1期62-69,共8页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(41271320);中央高校基本科研业务费专项(2017PY055)~~

摘　　要：为探究L-天冬氨酸纳米钙颗粒[Ca(L-asp)-NPs]进入环境后对棉花生长的影响,以鄂抗8号棉花为试验材料,设置0(对照)、25、50、75、100 mg·L^-1共计5个Ca(L-asp)-NPs处理浓度,于华中农业大学盆栽场进行试验,测定干鲜质量、根系形态、钙含量、叶绿素、抗氧化酶、MDA、电导率等指标。结果表明:与对照相比,Ca(L-asp)-NPs处理显著降低了棉花的干鲜质量并抑制根系伸长。根、茎、叶钙含量最大值为7905、13 144、38 117 mg·kg^-1,比对照增加了102%、61%、51%,但钙的转移因子在施入Ca(Lasp)-NPs后显著降低。外源Ca(L-asp)-NPs也显著降低了叶片叶绿素含量以及根系抗氧化酶活性和电导率,除25 mg·L^-1处理外,MDA含量也显著下降。研究表明,Ca(L-asp)-NPs进入环境后会抑制棉花的生理生长,对棉花生长产生抑制作用,同时棉花本身会提升根冠比来适应此胁迫。To investigate the effect of calcium L-aspartate nanoparticles[Ca(L-asp)-NPs] on cotton growth after entering the environment,using cotton(E′kang 8)as the experimental material, and a total of 5 Ca(L-asp)-NPs concentrations set at 0, 25, 50, 75 mg·L^-1 and 100 mg·L^-1. The pot experiment was carried out in the greenhouse of Huazhong Agricultural University. The dry and fresh weights, root morphological parameters, Ca content, chlorophyll, root antioxidant enzymes, malondialdehyde(MDA), and electrical conductivity were determined. Compared with the control, Ca(L-asp)-NPs treatments significantly reduced the dry and fresh weights of cotton and inhibited root elongation. The maximum Ca content in root, stem and leaves were 7905, 13 144 mg·kg^-1 and 38 117 mg·kg^-1, respectively, which increased by 102%, 61% and 51%, respectively when compared with the control;however the Ca transfer factor decreased significantly after Ca(L-asp)-NPs application. In addition, exogenous Ca(L-asp)-NPs also significantly reduced chlorophyll content, root antioxidant enzymes activity, and electrical conductivity;in addition, MDA content was also significantly decreased except in 25 mg·L^-1 treatment. In conclusion, it was determined that Ca(L-asp)-NPs can inhibit the physiological activity of cotton and further inhibit cotton growth and that cotton will increase its root-shoot ratio to adapt to stress.

关 键 词：棉花 叶绿素 L-天冬氨酸纳米钙 抗氧化酶 

分 类 号：X171.5[环境科学与工程—环境科学]