人参皂苷Rg1、Rb1抗Aβ人血管内皮细胞损害作用的研究  

Study on the effect of ginsenoside Rg1 and Rb1 for the anti-damage of Aβ on human vascular endothelial cells

作　　者：刘南海[1] 黄晓峰[2] 王振凤 章健 符雪涛 薛寿儒[7] 朱长东 杨凯[4] 罗江洪[5] LIU Nan-hai;HUANG Xiao-feng;WANG Zhen-feng;ZHANG Jian;FU Xue-tao;XUE Shou-ru;ZHU Chang-dong;YANG Kai;LUO Jiang-hong(Gannan Medical University The First Affiliated Hospital,Ganzhou,Jiangxi 341000;Gannan Medical University Nursing College,Ganzhou,Jiangxi 341000;Gannan Medical University The Second Affiliated Hospital,Ganzhou,Jiangxi 341000;Gannan Medical University pharmacy college;Gannan Medical University Basic Medical College,Ganzhou,Jiangxi 341000;Nanchang County People's Hospital,Nanchang,Jiangxi 330000;The First Affiliated Hospital of Soochow University,Suzhou,Jiangsu 215000;Ganzhou Municipal Hospital,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院.第一附属医院,江西赣州341000 [2]赣南医学院护理学院,江西赣州341000 [3]赣南医学院第二附属医院,江西赣州341000 [4]赣南医学院药学院,江西赣州341000 [5]赣南医学院基础医学院,江西赣州341000 [6]南昌县人民医院,江西南昌330000 [7]苏州大学附属第一医院,江苏苏州215000 [8]赣州市立医院,江西赣州341000

出　　处：《赣南医学院学报》2019年第12期1197-1200,1240,共5页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：江西省教育厅科学技术研究项目(GJJ150960);赣南医学院产学研项目(CP201407)

摘　　要：目的:探讨人参皂苷Rg1、Rb1对β-淀粉样蛋白(Aβ)诱导人脑血管内皮细胞增殖抑制情况的影响。方法:将人脑血管内皮细胞分为对照组、Aβ1-42组(20μmol·L^-1)、低人参皂苷Rg1组(20μmol·L^-1 Aβ1-42+Rg1 0.1μmol·L^-1)、中人参皂苷Rg1组(20μmol·L^-1 Aβ1-42+Rg1 1μmol·L^-1)、高人参皂苷Rg1组(20μmol·L^-1 Aβ1-42+Rg1 10μmol·L^-1)、低人参皂苷Rb1组(20μmol·L^-1 Aβ1-42+Rb1 0.1μmol·L^-1)、中人参皂苷Rb1组(20μmol·L^-1 Aβ1-42+Rb1 1μmol·L^-1)、高人参皂苷Rb1组(20μmol·L^-1 Aβ1-42+Rb1 10μmol·L^-1)。用MTT检测各组细胞增殖能力,Western-blot检测各组细胞中PCNA、Ki67、COX-2蛋白表达情况,ELISA法检测各组细胞中IL-1、TNF-α水平。结果:与对照组相比,Aβ1-42组细胞的增殖率、PCNA、Ki67蛋白表达显著降低(P<0.05),COX-2、IL-1、TNF-α水平显著升高,与Aβ1-42组细胞相比,低、中、高人参皂苷Rg1和低、中、高人参皂苷Rb1组细胞的增殖率、PCNA、Ki67蛋白表达显著升高(P<0.05),COX-2、IL-1、TNF-α水平显著降低(P<0.05)。结论:人参皂苷Rg1、Rb1可能是通过促进增殖蛋白及抑制炎症因子表达来改善Aβ导致的人脑血管内皮细胞增殖抑制。Objective: To explore effects of ginsenoside Rg1 and Rb1 on proliferation inhibition of β-amyloid protein(Aβ)-induced human brain microvascular endothelial cells. Methods: Human brain microvascular endothelial cells were divided into control group, Aβ1-42 group(20 μmol·L^-1), low ginsenoside Rg1 group(20 μmol·L^-1 Aβ1-42+Rg1 0.1 μmol·L^-1), medium ginsenoside Rg1 group(20 μmol·L^-1 Aβ1-42+Rg1 1 μmol·L^-1), high ginsenoside Rg1 group(20 μmol·L^-1 Aβ1-42+Rg1 10 μmol·L^-1), low ginsenoside Rb1 group(20 μmol·L^-1 Aβ1-42+Rb1 0.1 μmol·L^-1), medium insenoside Rb1 group(20 μmol·L^-1 Aβ1-42+Rb1 1 μmol·L^-1), and high ginsenoside Rb1 group(20 μmol·L^-1 Aβ1-42+Rb1 10 μmol·L^-1). The cell proliferation ability in each group was detected by MTT. The expression of proliferating PCNA, Ki67 and COX-2 protein in each group was detected by Western-blot. The levels of IL-1 and TNF-α in each group were detected by ELISA. Results: Compared with control group, proliferation rate, expression of PCNA and Ki67 protein in Aβ1-42 group were significantly decreased(P<0.05), while levels of COX-2, IL-1 and TNF-α were significantly increased. Compared with Aβ1-42 group, proliferation rate, expression of PCNA and Ki67 protein in low, medium, high ginsenoside Rg1 group, and low, medium, high ginsenoside Rb1 group were significantly increased(P<0.05), while levels of COX-2, IL-1 and TNF-α were significantly decreased(P<0.05). Conclusions: Ginsenoside Rg1 and Rb1 may improve proliferation inhibition of human brain microvascular endothelial cells induced by Aβ in the way of promoting proliferation proteins and inhibiting expression of inflammatory factors.

关 键 词：人血管内皮细胞 Β-淀粉样蛋白 人参皂苷 增殖 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]