GPC-3、CD10、CD147、CD138免疫组织化学联合检测 对于高分化肝细胞癌与异型增生的鉴别诊断价值  

Differential diagnostic value of the combined immunohistochemistry detection of GPC-3,CD10,CD147 and CD138 in highly differentiated hepatocelluar carcinoma and dysplasia

作　　者：刘杜先 刘兰侠 张杰东 LIU Du-xian;LIU Lan-xia;ZHANG Jie-dong(Department of Pathology,Nanjing Hospital Affiliated to Nanjing University of Traditional Medicine,The Second Hospital of Nanjing,Nanjing Jiangsu 210003,China)

机构地区：[1]南京中医药大学附属南京医院,江苏省南京市第二医院病理科,210003

出　　处：《蚌埠医学院学报》2019年第12期1660-1663,共4页Journal of Bengbu Medical College

基　　金：江苏省南京市卫计委一般性课题(YKK15143)。

摘　　要：目的:探讨磷脂酰肌醇蛋白聚糖-3(GPC-3)、CD10、CD147和CD138在高分化肝细胞癌与异型增生病理鉴别诊断中的联合互补诊断价值。方法:应用免疫组织化学法检测76例高分化肝细胞癌和癌周异型增生结节内GPC-3、CD10、CD147和CD138的表达情况并进行分析。结果:高分化肝细胞癌组织中GPC-3、CD10、CD147较异型增生结节内高表达,且异型增生结节内表达常常杂乱不均;而CD138在异型增生结节中高表达,高分化肝细胞癌组织中弱表达或不表达。高分化肝细胞癌和异型增生结节组织内GPC-3、CD10、CD147和CD138的表达差异均有统计学意义(P<0.01)。4种标记联合检测诊断高分化肝细胞癌的灵敏度达96.05%,而特异性仅为52.6%。结论:GPC-3、CD10、CD147和CD138对高分化肝细胞癌与异型增生具有较高的病理鉴别诊断价值,可作为肝穿标本内两者区别的客观辅助指标。Objective:To investigate the differential diagnostic value of the combined immunohistochemistry detection of GPC-3,CD10,CD147 and CD138 in highly differentiated hepatocelluar carcinoma and dysplasia.Methods:The levels of GPC-3,CD10,CD147 and CD138 in 76 cases with highly differentiated hepatocelluar carcinoma and peripheral dysplastic nodules were explored using immunohistochemistry,and the result of which was analyzed.Results:Compared with the dysplastic nodules,the levels of GPC-3,CD10 and CD147 in highly differentiated hepatocelluar carcinoma tissue were higher,and these expressions in dysplastic nodules were often disorder and uneven.The expression levels of CD138 in dysplastic nodules and highly differentiated hepatocelluar carcinoma tissue were high and weak or no,respectively.The differences of the levels of GPC-3,CD10,CD147 and CD138 were statistically significant between highly differentiated hepatocelluar carcinoma tissue and dysplastic nodule(P<0.01).The sensitivities of the combined detection of four markers in highly differentiated hepatocelluar carcinoma tissue and dysplastic nodule were 96.05%and 52.6%,respectively.Conclusions:The pathologic differential diagnosis value of the combined detection of GPC-3,CD10,CD147 and CD138 in highly differentiated hepatocellular carcinoma and dysplasia is higher,which can be used as the objective auxiliary indicators between the two in liver biopsy specimens.

关 键 词：肝细胞肝癌 异型增生结节 磷脂酰肌醇蛋白多糖-3 CD 147 CD10 CD138 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]