Study on Differentiation of Growing Points in Corn Sprouts into Multiple Stems  

作　　者：Mengyu LYU Fushuang DONG Jianfang CHAI Shuo ZHOU He ZHAO Yongwei LIU Fan YANG Xiaoyu LI Junmin ZHANG Haibo WANG 

机构地区：[1]Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051,China

出　　处：《Agricultural Biotechnology》2020年第1期43-44,共2页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of Hebei Province(C2017301071);Financial Project of Hebei Province(2017039339)

摘　　要：[Objectives] This study was conducted to improve the efficiency of genetic transformation using growing points of corn sprouts. [Methods] A mixed liquid of plant hormones 6-BA and KT at a suitable concentration and ratio was added dropwise to the growing points of corn sprouts, followed by culture under appropriate conditions. [Results] Corn sprouts could be induced to form multiple plants, thereby improving the transformation efficiency of growing points in corn sprouts. [Conclusions] This study provides a breeding method using growing points of corn sprouts as transformation receptors for direct plantlet formation without tissue culture by the Agrobacterium transgenic technology.[Objectives] This study was conducted to improve the efficiency of genetic transformation using growing points of corn sprouts. [Methods] A mixed liquid of plant hormones 6-BA and KT at a suitable concentration and ratio was added dropwise to the growing points of corn sprouts, followed by culture under appropriate conditions. [Results] Corn sprouts could be induced to form multiple plants, thereby improving the transformation efficiency of growing points in corn sprouts. [Conclusions] This study provides a breeding method using growing points of corn sprouts as transformation receptors for direct plantlet formation without tissue culture by the Agrobacterium transgenic technology.

关 键 词：CORN Sprout growing point DIFFERENTIATION PLANT 

分 类 号：S513[农业科学—作物学]