五味子多糖对2型糖尿病大鼠血清中炎症因子的影响及其作用机制  

Effect of Schisandra chinensis polysaccharide on serum inflammatory factors in T2DM rats and its mechanism

作　　者：杜兴旭[1] 乔子敬 杨硕 许智莹 杨波 李贺[2] 陈建光[2] 王春梅[2] DU Xingxu;QIAO Zijing;YANG Shuo;XU Zhiying;YANG Bo;LI He;CHEN Jianguang;WANG Chunmei(Department of Endocrinology,Affiliated Hospital,Beihua University,Jilin 132002,China;Department of Pharmacology,College of Pharmacy,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学附属医院内分泌科,吉林吉林132002 [2]北华大学药学院药理学教研室,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2020年第1期50-55,I0003,共7页Journal of Jilin University：Medicine Edition

基　　金：吉林省教育厅“十三五”科学技术项目资助课题(JJKH20180375KJ);吉林省发改委省级产业创新专项资金资助课题(2018C046-2)

摘　　要：目的:观察五味子多糖(SCP)对高脂饮食联合小剂量链脲佐菌素(STZ)诱导的2型糖尿病(T2DM)模型大鼠血清中炎症因子的影响,并探讨其治疗T2DM的可能机制。方法:雄性Wistar大鼠给予高脂饮食喂养,并一次性腹腔注射小剂量STZ (30mg·kg^-1)建立T2DM模型。将造模成功的大鼠随机分为模型组、低剂量(25mg·kg^-1)SCP组、中剂量(50mg·kg-1)SCP组和高剂量(100mg·kg^-1)SCP组,每组10只,另取10只健康大鼠设为正常对照组。灌胃治疗8周后,各组大鼠行口服葡萄糖耐量试验(OGTT),采用葡萄糖氧化酶法检测空腹血糖(FBG),放射免疫分析法检测空腹血胰岛素(INS)水平并计算胰岛素敏感指数(ISI),采用酶联免疫吸附法(ELISA)检测大鼠血清中白细胞介素6 (IL-6)、C反应蛋白(CRP)、白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)和核因子-κB (NF-κB)水平,HE染色观察大鼠胰腺组织病理形态表现。结果:与正常对照组比较,模型组大鼠FBG明显升高(P<0.01),血糖曲线下面积(AUC)明显增加,INS水平和ISI明显降低(P<0.05或P<0.01),血清中IL-6、CRP、IL-1β、TNF-α和NF-κB水平均明显升高(P<0.05或P<0.01)。与模型组比较,不同剂量SCP组大鼠FBG水平明显降低(P<0.05),血糖AUC明显下降,INS水平和ISI明显升高(P<0.05),血清中IL-6、CRP、IL-1β、TNF-α和NF-κB水平均明显降低(P<0.05或P<0.01)。与低剂量SCP组比较,中和高剂量SCP组大鼠FBG水平明显降低(P<0.05),高剂量SCP组大鼠血清中INS水平明显升高(P<0.05)。HE染色,与正常对照组比较,模型组大鼠胰岛萎缩,胰岛内细胞数目减少,边界不整;与模型组比较,不同剂量SCP组大鼠胰岛边界逐渐清晰,面积增大,细胞数目明显增加。结论:SCP可降低高脂饮食联合小剂量STZ诱导的T2DM大鼠FBG水平,升高INS水平,改善胰岛素抵抗(IR),其作用机制可能与抑制机体的炎症反应有关。Objective:To observe the effect of Schisandra Chinensis polysaccharide(SCP)on the serum inflammatory factors in the rats with type 2 diabetes mellitus(T2DM)induced by high-fat diet and low dose of streptozotocin(STZ),and to explore its underlying mechanism in the treatment of T2DM.Methods:The male Wistar rats were given high-fat diet and introperieoneally injected with low dose of STZ(30 mg·kg-1)in one time to establish the rat T2DM models.The successful model rats were randomly divided into model group,low dose(25 mg·kg^-1)of SCP group,middle dose(50 mg·kg^-1)of SCP group and high dose(100 mg·kg^-1)of SCP group;there were 10 rats in each group.Another 10 healthy rats were used as normal control group.Eight weeks after the intragastric administration of SCP,oral glucose tolerance test(OGTT)was performed in the rats of various groups.The fasting blood glucose(FBG)and insulin(INS)levels were detected by glucose oxidase method and radioimmunoassay method,respectively,and the insulin sensitivity index(ISI)was calculated.The levels of interleukin-6(IL-6),C-reactive protein(CRP),interleukin-1β(IL-1β),tumor necrosis factor-α (TNF-α)and nuclear factor-κB(NF-κB)in serum of the rats were measured by enzyme linked immunosorbent assay(ELISA)method.HE staining was used to observe the pathomorphology of pancreas tissue of the rats.Results:Compared with normal control group,the serum FBG level of the rats in model group was significantly increased(P<0.01),and the area under the curve(AUC)of blood glucose of the rats was significantly increased,the serum INS level and the ISI were significantly decreased(P<0.05 or P<0.01);the levels of IL-6,CRP,IL-1β,TNF-α,and NF-κB in serum were all significantly increased(P<0.05 or P<0.01).Compared with model group,the serum FBG levels of the rats in different doses of SCP groups were markedly decreased(P<0.05),the AUC of blood glucose of the rats were significantly decreased,the INS and the ISI levels were significantly elevated(P<0.05);the levels of IL-6,CRP,IL-1β,TNF-αand NF-

关 键 词：五味子多糖 2型糖尿病 炎症因子 高脂饮食 链脲佐菌素 空腹血糖 

分 类 号：R587.1[医药卫生—内分泌]