食源性原花青素对SH-SY5Y细胞生长的抑制作用及其机制  

Inhibitory effect of foodborne procyanidins on growth of SH-SY5Y cells and its mechanism

作　　者：钟越 李海超[2] 冯馨锐 崔雨舒 郑中华[2] 王玮瑶[2] 齐玲[2,3] ZHONG Yue;LI Haichao;FENG Xinrui;CUI Yushu;ZHENG Zhonghua;WANG Weiyao;QI Ling(Faculty of Management,Jilin Medical University,Jilin 132013,China;Department of Pathophysiology,Jilin Medical University,Jilin 132013,China;Sixth Affiliated Hospital,Guangzhou Medical University,Qingyuan People’s Hospital,Guangdong Province,Qingyuan 511518,China)

机构地区：[1]吉林医药学院管理学院,吉林吉林132013 [2]吉林医药学院病理生理学教研室,吉林吉林132013 [3]广州医科大学附属第六医院广东省清远市人民医院,广东清远511518

出　　处：《吉林大学学报（医学版）》2020年第1期61-65,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(20160101195JC,20180101306JC,20190101006JH);吉林省卫健委科研项目资助课题(2016J102)

摘　　要：目的:探讨食源性原花青素对人神经母细胞瘤SH-SY5Y细胞生长的抑制作用,并阐明其作用机制。方法:培养SH-SY5Y细胞,将细胞分为对照组及10、20和40mg·L^-1食源性原花青素组,各组细胞中加入含不同浓度(0、10、20和40mg·L^-1)食源性原花青素的培养基,采用噻唑蓝(MTT)法检测药物作用24、48和72h时SH-SY5Y细胞增殖率,流式细胞术检测药物作用72h时不同细胞周期SH-SY5Y细胞百分率,AnnexinⅤ凋亡试剂盒检测药物作用72h时SH-SY5Y细胞的凋亡率。结果:与对照组比较,各时间点10、20和40mg·L^-1食源性原花青素组SH-SY5Y细胞增殖率均降低,作用48和72h时20mg·L^-1食源性原花青素组细胞增殖率差异有统计学意义(P<0.05或P<0.01),作用24、48和72h时40mg·L^-1食源性原花青素组细胞增殖率差异有统计学意义(P<0.01)。与对照组比较,40mg·L^-1组食源性原花青素组细胞中G0/G1期细胞百分率升高(P<0.01),G2/M期细胞百分率降低(P<0.01)。与对照组比较,10、20和40mg·L^-1食源性原花青素组细胞凋亡率均明显升高(P<0.01)。结论:食源性原花青素可抑制人神经母细胞瘤SH-SY5Y细胞的生长,其机制主要是阻滞细胞周期和诱导细胞凋亡。Objective:To investigate the inhibitory effect of foodborne procyanidins on the growth of human neuroblastoma SH-SY5 Ycells,and to elucidate its mechanism.Methods:The SH-SY5 Ycells were cultured and divided into control group,10 mg·L^-1 foodborne procyanidins group,20 mg·L^-1 foodborne procyanidins group and 40 mg·L^-1 foodborne procyanidins group;the medium containing different concentrations(0,10,20 and40 mg·L^-1)of foodborne procyanidins was added into each group.The proliferation rates of SH-SY5 Ycells were measured by MTT method at 24,48 and 72 hafter the drug treatment.Flow cytometry was used to detect the cell cycle of SH-SY5 Ycells at 72 hafter the drug treatment,and the apoptotic rate of SH-SY5 Ycells was detected by Annexin Ⅴ apoptotic assay kit at 72 hafter the drug treatment.Results:Compared with control group,the proliferation rates of SH-SY5 Ycells at 24,48 and 72 hin 10,20 and 40 mg·L^-1 foodborne procyanidins groups were decreased;there were significant differences at 48 and 72 hours in 20 mg·L^-1 foodborne procyanidins group(P<0.05 or P<0.01);there were also significant differences at 24,48 and 72 hin 40 mg·L^-1 foodborne procyanidins group(P <0.01).Compared with control group,the percentage of cells in G0/G1 phase in40 mg·L^-1 foodborne procyanidins group were increased(P<0.01)and the percentage of cells in G2/M phase were decreased(P<0.01).Compared with control group,the apoptotic rates of cells in 10,20 and 40 mg·L^-1 foodborne procyanidins groups were increased significantly(P<0.01).Conclusion:Foodborne procyanidins can inhibit the growth of human neuroblastoma SH-SY5 Ycells,and its mechanism is mainly to block the cell cycle and induce the apoptosis.

关 键 词：食源性原花青素 SH-SY5Y细胞 细胞周期 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]