Eco-friendly preparation of chitooligosaccharides with different degrees of deacetylation from shrimp shell waste and their effects on the germination of wheat seeds  

作　　者：Xiaodan Fu Lin Zhu Li Li Tan Zhang Meng Li Haijin Mou 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,No.5 Yushan Road,Qingdao 266003,China

出　　处：《Marine Life Science & Technology》2019年第1期95-103,共9页海洋生命科学与技术（英文）

摘　　要：Production of chitosan and its derivatives by traditional methods involves the excessive use of a reaction solution comprisedof sodium hydroxide and hydrochloric acid. Waste water resulting from this process has lim让ed the application of chitosanas a fertilizer as the process causes serious environmental pollution. Specifically, the resulting waste water contains highlevels of dissolved nitrogen and minerals from shrimp shells. In this study, an eco-friendly method was established to produce chitooligosaccharides (COS) with different degrees of deacetylation (DDAs) from shrimp shell waste. At a solid-tosolventratio of 1:6, the degree of demineralization was above 90% with the treatment of 30 g-L_1 H3PO4, and the degree ofdeproteinization was above 80% when treated with 30 g-L_1 KOH at 70 °C. Chitosans with different DDAs were obtainedby microwave-assisted KOH metathesis and the COS with Mw approximately 1500 Da were then prepared by oxidativedegradation. In summary, 33.73 kg H3PO4,12.77 kg, and 241.31 kg KOH were supplied during the processes of demineralization,deproteinization, and deacetylation of 100 kg shrimp shell waste, respectively. The process water was totally recycled,demonstrating that the shrimp shell could be wholly transformed into fertilize The entire process created a product withthe fractions of N:P2O5:K2O:COS = 7.94:24.44:10.72:1 &27. The test on the germination promotion of wheat seeds revealedthat the COS with 72」2% DDA significantly promoted germination. This work demonstrated the use of an eco-friendlypreparation method of COS with a specific degree of deacetylation that can be applied as a fertilizer.

关 键 词：CHITOOLIGOSACCHARIDE Deacetylation degree Phosphoric acid Potassium hydroxide Germination promotion 

分 类 号：G63[文化科学—教育学]