丹防胶囊对小鼠非酒精性脂肪性肝纤维化的干预效果及作用机制  

Effect of Danfang Capsules on Non-alcoholic Fatty Liver Disease in Mice

作　　者：李璨 陆爽[1] 吴君[1] LI Can;LU Shuang;WU Jun(Research and Teaching Office of Infectious Diseases,Guizhou Medical College,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学感染病学教研室,贵州贵阳550004

出　　处：《贵州医科大学学报》2020年第1期61-66,共6页Journal of Guizhou Medical University

基　　金：贵阳市科技计划项目(201120419)

摘　　要：目的:探讨丹防胶囊对小鼠非酒精性脂肪性肝纤维化的干预效果及机制。方法:30只雄性C57BL/6J小鼠均分为正常组、模型组及丹防组,正常组给予普通饮食,模型组及丹防组给予高脂饮食喂养8周建立非酒精性脂肪性肝纤维化模型;造模同时给予丹防组小鼠1.22 g/20 g丹防胶囊灌胃,另2组小鼠给予等量生理盐水灌胃,1次/d,共8周;第8周记录小鼠体质量,检测血糖、甘油三酯(TG)、胆固醇(TC)、谷丙转氨酶(ALT)、谷草转氨酶(AST)水平;第8周末麻醉处死小鼠取肝脏组织记录肝脏重量,观察组织学改变,检测TGF-β1、Smad2、α-平滑肌(α-SMA)蛋白和mRNA及Ⅳ型胶原(col-Ⅳ)mRNA表达水平。结果:与正常组比较,模型组小鼠体质量、肝脏重量、血糖、TG、TC、ALT、AST水平均显著升高(P<0.05),肝组织空泡形成明显,有大量红色脂滴蓄积、肝纤维化严重,肝脏组织TGF-β1、Smad2及α-SMA蛋白表达水平、col-ⅣmRNA表达水平均显著上升(P<0.05);与模型组比较,丹防组小鼠体质量、血糖、TG、TC水平无明显变化(P>0.05),肝脏重量、ALT及AST水平显著下降(P<0.05),肝组织空泡形成及脂滴蓄积减少,肝纤维化程度减轻,肝组织TGF-β1、Smad2、α-SMA蛋白表达水平、col-ⅣmRNA表达水平下降(P<0.05)。结论:丹防胶囊可降低非酒精性脂肪性肝纤维化肝脏重量、ALT、AST水平,其机制可能与降低肝组织中TGF-β1及Smad2的表达有关。Objective:To investigate the effect and mechanism of Danfang capsules on non-alcoholic fatty liver disease(NAFLD)in mice.Methods:30 male C57BL/6J mice were randomly divided into the normal group,the model group and Danfang group,10 mice in each group.The normal group was fed with normal diet,and the model group with high-fat diet for 8 weeks to establish the model with NAFLD.At the same time,1.22 g/20 g Danfang capsules were given to Danfang group,and other groups were given the same amount of normal saline once a day for 8 weeks.The body weight,the level of blood glucose,triglyceride(TG),cholesterol(TC),ALT and AST were measured during the 8th week and mice were sacrificed under anesthesia and their livers were weighed.Liver histopathological examination was performed.The expression levels of TGF-β1,Smad2,α-SMA and col-ⅣmRNA in liver tissues were detected by Western blot and RT-qpcr.Results:Compared with the normal group,the body weight,liver weight,blood glucose,TG,TC,ALT,AST levels in the model group increased(all P<0.05),and the liver tissue vacuoles were significantly formed,with a large amount of red lipid accumulation and severe liver fibrosis.The liver tissue TGF-β1,Smad2,α-SMA expression and col-ⅣmRNA levels increased(P<0.05).Compared with the model group,there were no significant changes in body weight,blood glucose,TG and TC levels in Danfang group(P>0.05),but the liver weight,ALT and AST levels were reduced(P<0.05).The liver vacuolation,lipid droplet accumulation and degree of liver fibrosis decreased.The expression of TGF-β1,Smad2,α-SMA and col-ⅣmRNA levels in liver tissues decreased(P<0.05).Conclusion:Danfang capsules can reduce the liver weight,ALT and AST levels of NAFLD.The mechanism may be related to the reduction of the expression of TGF-β1 and Smad2 in liver tissues.

关 键 词：丹防胶囊 非酒精性脂肪性肝纤维化 TGF-Β1 SMAD2 Ⅳ型胶原蛋白 α-平滑肌蛋白 

分 类 号：R575.2[医药卫生—消化系统]