口腔鳞状细胞癌组织CIRP和TLR4蛋白表达与患者临床病理特征及预后的关系  

Relationship between Expression of CIRP and TLR4 in Oral Squamous Cell Carcinoma and Clinic-pathological Features and Prognosis

作　　者：张琳梅 高岭[2] 李少明[2] 陈诚[3] ZHANG Linmei;GAO Lin;LI Shaoming;CHEN Cheng(Stomatology College of Xi'an Medical University,Xi'an 710021,Shaanxi,China;Department of Maxillofacial Surgey,the Affiliated Hospital of Qingdao University,Qingdao 266555,Shandong,China;Department of General Dentistry,Xi'an Jiaotong University Stomatological Hospital,Xi'an 710004,Shaanxi,China)

机构地区：[1]西安医学院口腔医学院,陕西西安710021 [2]青岛大学附属医院口腔颌面外科,山东青岛266555 [3]西安交通大学口腔医院口腔综合科,陕西西安710004

出　　处：《贵州医科大学学报》2020年第1期91-96,共6页Journal of Guizhou Medical University

基　　金：陕西省教委基金(19JK0764)

摘　　要：目的:探讨口腔鳞状细胞癌(OSCC)组织中冷诱导RNA结合蛋白(CIRP)和Toll样受体4(TLR4)的表达与患者临床病理特征及预后的关系。方法:选取确诊的40例OSCC患者癌组织标本作为研究组、瘤周正常组织作为对照组;收集患者的临床病理特征,采用免疫组织化学染色检测CIRP和TLR4蛋白的表达;电话随访患者或其家属,收集死亡患者的生存时间,采用Kaplan-Meier法分析死亡患者CIRP和TLR4表达对预后的影响。结果:研究组CIRP和TLR4蛋白阳性检出率均明显高于对照组,差异均有高度统计学意义(P<0.01);癌组织CIRP蛋白高、低表达组的患者TNM分期、临床分期、组织病理学分期及淋巴结转移的差异均有统计学意义(P<0.05),癌组织TLR4高、低表达组患者的性别、TMN分期、临床分期、组织病理学分级以及淋巴结转移的差异均有统计学意义(P<0.05);随访结果显示,癌组织CIRP高表达患者术后的生存率明显低于低表达患者(χ2=6.651,P=0.009),癌组织TLR4高表达患者术后的生存率明显低于TLR4低表达患者(χ2=8.473,P=0.004)。结论:CIRP与TLR4在OSCC癌组织中呈高表达,并与患者TNM分期、临床分期、组织病理学分期、淋巴结转移以及较差的生存率有关联。Objective:To investigate the expression of cold induced RNA combined protein(CIRP)and Toll-like receptor 4(TLR4)of oral squamous cell carcinoma(OSCC)and its relationship with clinic-pathological features and prognosis.Methods:Selecting 40 cases of OSCC patients sample as experiment group,peritumoral-normal tissue as control group;collecting clinic-pathological features of patients,adopting Immunohistochemical staining to evaluate the expression of CIRP and TLR4;phone call follow-up on patients or family to collect survival time of deceased patients.Adopting Kaplan-Meier assay to analyze effect of CIRP and TLR4 of deceased patients on prognosis.Results:The positive detection rate of CIRP and TLR 4 in the experiment group was higher than that of the control group,differences were highly statistically significant(P<0.01);the differences of TNM staging,clinical staging,histopathological staging and lymph node metastasis of of CIRP protein high and low expression groups were statistically significant(P<0.05);the differences of gender,TNM staging,clinical staging,histopathological staging and lymph node metastasis of of TLR4 high and low expression groups were statistically significant(P<0.05).The overall survival rate of patients with high CIRP expression was lower than that of patients with low expression(χ2=6.651,P=0.009),overall survival rate of patients with high TLR4 expression was lower than that of patients with low expression(χ2=8.473,P=0.004).Conclusion:CIRP and TLR4 showed high expression in OSCC patients,which was correlated with TNM staging,clinical staging,histopathological staging and lymph node metastasis as well as poor survival rate.

关 键 词：TOLL样受体4 口腔鳞状细胞癌 免疫组织化学 病理学 临床 冷诱导RNA结合蛋白 肿瘤预后 

分 类 号：R739.81[医药卫生—肿瘤]