利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性  

Assessment of genetic diversity by using EST-SSR markers in Lupinus

作　　者：张红岩[1,5] 杨涛[1] 刘荣[1] 晋芳[2] 张力科[2] 于海天[3] 胡锦国[4] 杨峰 王栋[1] 何玉华[3] 宗绪晓[1] ZHANG Hong-Yan;YANG Tao;LIU Rong;JIN Fang;ZHANG Li-Ke;YU Hai-Tian;HU Jin-Guo;YANG Feng;WANG Dong;HE Yu-Hua;ZONG Xu-Xiao(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China;National Agro-Tech Extension&Service Centre,Beijing 100125,China;Institute of Food Crops,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan,China;USDA-ARS Western Regional Plant Introduction Station(WRPIS),Pullman,WA 99164,USA;Qinghai University,Xining 810016,Qinghai,China)

机构地区：[1]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081 [2]全国农业技术推广服务中心,北京100125 [3]云南省农业科学院粮食作物研究所,云南昆明650205 [4]USDA-ARS Western Regional Plant Introduction Station(WRPIS),Pullman,WA 99164,USA [5]青海大学,青海西宁810016

出　　处：《作物学报》2020年第3期330-340,共11页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2017YFE0105100);农作物种质资源保护与利用专项(2019NWB036-07);中国农业科学院科技创新工程项目和中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项(S2018XC04)资助~~

摘　　要：利用基于窄叶羽扇豆转录组开发并筛选出的95对多态性EST-SSR标记,对羽扇豆属的22个种133份资源进行全基因组扫描,初步探究羽扇豆属下种间的进化关系,分析来源于“旧世界”羽扇豆种间的遗传多样性,为羽扇豆优异资源的挖掘和创新利用提供理论依据。结果表明,用95对SSR标记共检测出1318个等位变异,每对标记平均检测出3~37个等位变异,平均为13.87个;多态性信息量(PIC)变化范围0.39~0.91,平均为0.75;基因多样性变化范围0.41~0.92,平均为0.78。基于邻接法(NJ)的系统发育初步探究了羽扇豆种间的进化关系,22个羽扇豆种分别来源于“旧世界”和“新世界”,与之前研究结果相对一致。聚类分析、群体结构分析和主成分分析结果均表明,来源于“旧世界”的7个羽扇豆种被划分为4个组群,各组群所包含的参试资源没有种间交叉重叠。In order to explore the evolutionary relationship of Lupinus preliminarily as well as to excavate and utilize lupin resources from the“Old World”effectively,the genetic diversity among the species under Lupinus genus was analyzed.Ninety-five polymorphic pairs of EST-SSR markers developed based on the transcriptome of narrow-leaved lupin(Lupinus angustifolius L.)were used to scan 133 lupin accessions from 22 species.A total of 1318 alleles were detected with 13.87 alleles per locus on average,ranging from 3 to 37 alleles;the polymorphism information content(PIC)ranged from 0.39 to 0.91 with the mean value of 0.63;the genetic diversity ranged from 0.41 to 0.92 with the mean value of 0.78.This study showed evolutionary relations among the 22 species under Lupinus genus from the“Old World”and the“New World”based on Neighbor-Joining(NJ)method,which is consistent with previous studies.Moreover,seventy-seven lupin accessions of seven Lupinus species from the“Old World”were divided into 4 groups;there was no overlap of accession from different species contained in each identified group,detected by all the three analysis methods like population structure,cluster analysis based on UPGMA and principal component analysis(PCA).

关 键 词：EST-SSR 羽扇豆属 转录组 遗传多样性 

分 类 号：S681.9[农业科学—观赏园艺]