盐酸洛拉曲克通过调控caspase-3促进人肝癌细胞HepG-2凋亡  

Nolatrexed Dihydrochloride promotes human hepatocellular carcinoma cell HepG-2

作　　者：李顺延 高飞[2] 郭虎林 LI Shun-yan;GAO Fei;GUO Hu-Lin(The Fifth People's Hospital of Qinghai Province,Xining,Qinghai 810007,China;The Second Affiliated Hospital of Dalian Medical University,Dalian,Liaoning 116031,China)

机构地区：[1]青海省第五人民医院,1810007 [2]大连医科大学附属第二医院,116031

出　　处：《现代消化及介入诊疗》2020年第1期11-15,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：大连市医学科学研究计划项目(1712033)

摘　　要：目的探讨盐酸洛拉曲克对人肝癌细胞HepG-2凋亡的影响及作用机制。方法用不同梯度浓度的NLTX作用于人肝癌细胞HepG-2后,检测NLTX对HepG-2细胞凋亡及凋亡相关蛋白Bcl-2、Bax、caspase-3表达的影响。结果NLTX能抑制人肝癌细胞HepG-2增殖并诱导人肝癌细胞HepG-2凋亡,且呈浓度依赖性;NLTX可下调HepG-2细胞Bcl-2表达、上调Bax表达及增强caspase-3的活性,促进pro-caspase3向caspase成熟,但对caspase-3的mRNA的转录和翻译无明显影响。结论NLTX可抑制人肝癌细胞HepG-2的增殖,诱导人肝癌细胞HepG-2凋亡,其作用机制可能是通过上调人肝癌细胞HepG-2内Bax蛋白的表达及促进caspase-3的成熟,增强caspase-3的活性而发挥作用。Objective To investigate the effect and mechanism of nolatrexed dihydrochloride(NLTX)on apoptosis of human hepatocellular carcinoma cell HepG-2.Methods The effects of NLTX on HepG-2 and bcl-2 Bax caspase-3 expression in human hepatocellular carcinoma cells were detected by treating HepG-2 cells with different gradient concentrations of NLTX.Results NLTX could inhibit HepG-2 proliferation and induce HepG-2 apoptosis of human liver cancer cells in a concentration-dependent manner.NLTX could down-regulate bcl-2 expression in HepG-2 cells,up-regulate Bax expression,enhance the activity of caspase-3,and promote the maturation of pro-caspase-3 to caspase,but had no significant effect on the transcription and translation of caspase-3 mRNA.Conclusion NLTX can induce the apoptosis of human hepatocellular carcinoma cell HepG-2,probably through promoting the maturity of caspase-3 and increasing its activity.

关 键 词：肝癌 盐酸洛拉曲克 BAX CASPASE-3 

分 类 号：R735.7[医药卫生—肿瘤] R575[医药卫生—临床医学]