恒河猴肺中阴沟肠杆菌的分离鉴定及药物敏感性分析  

Isolation,identification,and drug sensitivity analysis of Enterobacter cloacae from rhesus monkey lung

作　　者：李艳艳[1] 禹文海[1] 杨凤梅[1] 刘权 陈丽雄[1] 王俊斌[1] 马进[1] 徐鸿界 杨亚平 赵远[1] 和占龙[1] LI Yanyan;YU Wenhai;YANG Fengmei;LIU Quan;CHEN Lixiong;WANG Junbin;MA Jin;XU Hongjie;YANG Yaping;ZHAO Yuan;HE Zhanlong(Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases,Kunming 650118,China)

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室

出　　处：《中国比较医学杂志》2020年第1期68-73,共6页Chinese Journal of Comparative Medicine

基　　金：中国医学科学院医学与健康科技创新工程项目(2016-I2M-2-001,2018-I2M-3-002);云南省科技创新人才计划(2015HC027);昆明市科技创新和服务能力提升计划重点项目(2016-2-R-07674)

摘　　要：目的对死亡的恒河猴肺脏炎症组织采样进行病原菌分离、培养、鉴定及药敏试验,确诊病原菌及药物敏感性,指导疾病诊疗、预防和临床用药。方法解剖柜条件下解剖死亡猴,分别采集肉眼病变明显和正常肺组织,用灭菌接种环在肺切面部位采样,接种在SS琼脂培养基进行培养,培养时间24 h观察细菌生长情况,进行生化鉴定和8种药敏试验。结果菌落呈白色或乳白色、圆形、湿润、不透明,进一步分离培养,经梅里埃微生物生化鉴定,确定该细菌为阴沟肠杆菌,ID:99.9%,T:0.71,ID编码:35074753311;药敏试验表明该菌对氨苄西林,头孢呋辛中等敏感;对诺氟沙星、阿米卡星、庆大霉素、头孢曲松、头孢哌酮,头孢噻肟敏感。动物实验表明该分离菌对实验小鼠具有致病性。结论该恒河猴肺部感染阴沟肠杆菌,头孢哌酮,头孢噻肟等药物可作为治疗临床用药。Objective To isolate,culture,identify,and analyze the drug sensitivity of pathogenic bacteria in inflamed lung tissue from a dead rhesus monkey,to determine the drug susceptibility of the bacteria,and to guide proper clinical application of such information.Methods The rhesus monkey was dissected under aseptic conditions.The lung was cut open and then insert focus of infection in the lung with inoculating loop to sampling and inoculating on plating medium.The presence of bacteria was observed,biochemical identification was performed,and eight kinds of antibiotic susceptibility test were carried out.Results Bacterial colonies on SS agar medium were white or ivory white,round,moist,and opaque.After the biochemical identification,the bacterium was identified as Enterobacter cloacae(ID:99.9%,T:0.71,ID number:35074753311).The drug sensitivity test showed that the susceptibility of Enterobacter cloacae to eight antimicrobial agents was as follows:ampicillin and cefuroxime,intermediate sensitivity;and norfloxacin,amikacin,gentamicin,cefatriaxone,cefoperazone,and cefotaxime,saitivity.The Enterobacter cloacae isolated from rhesus monkey also showed a high risk to Kunming mice.Conclusions We conclude that the rhesus monkey lung was infected with Enterobacter cloacae.Cefoperazone and cefotaxime can be used clinically to treat cases like this in rhesus monkey.

关 键 词：阴沟肠杆菌 恒河猴 分离鉴定 药敏试验 

分 类 号：R-33[医药卫生]