肺癌癌组织中M2-PK、和ENO1的表达水平及对其生存期的预测价值  

Expression levels of M2-PK and ENO1 in lung cancer tissues and their predictive value on survival

作　　者：范华颖[1] 张家艳[1] 方怡[2] 高阳[3] FAN Huaying;ZHANG Jiayan;FANG Yi;GAO Yang(Department of Respiratory Medicine,The third People􀆳s Hospital of Chengdu,Chengdu,Sichuan,China,610031;Department of Integrated Traditional Chinese and Western Medicine,West China Hospital,Sichuan University,Chengdu,Sichuan,China,610041;General medical center,Sichuan Provincial People􀆳s Hospital,Chengdu,Sichuan,China,610072)

机构地区：[1]成都市第三人民医院呼吸内科,四川成都610031 [2]四川大学华西医院中西医结合科,四川成都610041 [3]四川省人民医院全科医学中心,四川成都610072

出　　处：《分子诊断与治疗杂志》2020年第1期79-83,共5页Journal of Molecular Diagnostics and Therapy

基　　金：四川省卫生和计划生育委员会科研课题项目(18PJ524)

摘　　要：目的研究肺癌组织中肿瘤型丙酮酸激酶(M2-PK)和烯醇化酶-α(ENO1)的表达对患者生存期的预测价值。方法采用免疫组织化学染色法分别对182例肺癌患者和68例肺良性病变组织切片进行检测,观察M2-PK和ENO1在肺癌组织和良性病变组织中的表达情况,分析M2-PK和ENO1表达情况与肺癌临床病理特征及患者生存期的关系。结果肺癌组织中M2-PK和ENO1阳性表达率分别为78.02%、67.58%,高于良性病变组织的32.36%和29.41%,差异具有统计学意义(P<0.001),其表达与肺癌浸润分期(P=0.027,P=0.019)、分化程度(P=0.041,P=0.037)、淋巴转移(P=0.009,P=0.001)、TNM分期均显著相关(P=0.012,P=0.015);肺癌组织中M2-PK与ENO1表达呈正相关(r=0.769,P<0.05);肺癌组织中M2-PK和ENO1的高表达与患者5存活率分别为37.35%和32.31%,低于低表达的62.62%和52.99%,差异具有统计学意义(P<0.05);M2-PK与ENO1共高表达患者5年存活率为28.84%,低于共低表达的59.32%(P<0.05)。结论肺癌组织中M2-PK和ENO1的高表达与肺癌的发生、发展及预测患者的生存期有一定的相关性,可能存在临床应用价值。Objective To investigate the expression levels of tumor M2 pyruvate kinase(M2⁃PK)and enolase⁃α(ENO1)in lung cancer tissues and their predictive value on survival time of patients.Methods T Immunohistochemical staining was used to detect 182 lung cancer patients and 68 lung benign lesion tissue sections,respectively,to observe the expression of M2⁃PK and ENO1 in lung cancer tissue and benign lesion tissue,and to analyze the expression of M2⁃PK and ENO1.The relationship between the clinicopathological characteristics of lung cancer and the survival time of patients was analyzed.Results The positive expression rates of M2⁃PK and ENO1 in lung cancer tissues were 78.02%and 67.58%respectively,which were higher than those in benign lesion tissues with 32.36%and 29.41%(P<0.001,P<0.001).And their expression levels were significantly correlated with lung cancer infiltration staging(P=0.027,P=0.019),differentiation degree(P=0.041,P=0.037),lymphatic metastasis(P=0.009,P=0.001)and TNM staging(P=0.012,P=0.015).ENO1 expression was positively correlated with M2⁃PK expression in lung cancer tissues(r=0.769,P<0.05).The 5⁃year survival rates of patients with high expression of M2⁃PK or ENO1 in lung cancer tissues were lower than those in patients with low expression[(37.35%and 32.31%)vs(62.62%and 52.99%)](P<0.05).The 5⁃year survival rate of patients with high expression of ENO1 and M2⁃PK was lower than that in patients with low expression(28.84%vs 59.32%)(P<0.05).Conclusions The high expression of M2⁃PK and ENO1 in lung cancer tissues is related to the occurrence,development of lung cancer,and prediction of patient survival,which may have clinical application value.

关 键 词：肿瘤型丙酮酸激酶 烯醇化酶-α 肺癌 生物标志物 生存期预测 

分 类 号：R734.2[医药卫生—肿瘤]