沉默膜联蛋白A2对口腔细胞癌KB细胞迁移侵袭能力的影响  

The effect of silent membrane-linked protein A2 on the migration and invasion ability of KB cells in oral cell carcinoma

作　　者：麻颖宜 王海业 MA Ying-yi;WANG Hai-ye(Department of Periodonta,The First Affiliated Hospital of Zhengzhou University·Henan Stomatological Hospital,Zhengzhou,Henan 450052,China;不详)

机构地区：[1]郑州大学第一附属医院·河南省口腔医院牙周科,河南郑州450052 [2]郑州大学第一附属医院·河南省口腔医院特诊科,河南郑州450052

出　　处：《实用医药杂志》2020年第2期148-150,167,I0001,共5页Practical Journal of Medicine & Pharmacy

摘　　要：目的探讨沉默膜联蛋白A2(AA2)对口腔细胞癌KB细胞迁移侵袭能力的影响。方法转染siRNA-AA2至KB细胞沉默AA2的表达,PCR实验验证沉默效果,Transwell实验检测细胞侵袭的能力,划痕实验检测各组细胞迁移能力。结果与对照组比较,si-AA2组细胞中AA2表达量显著降低(P<0.05);与对照组相比,si-AA2组中KB细胞穿膜细胞数量显著低于对照组(P<0.05);划痕细胞24 h和48 h后,si-AA2组的愈合面积相对于对照组显著减少(P<0.05)。结论干扰AA2基因表达可抑制口腔细胞癌KB细胞迁移侵袭能力。Objecteve To explore the effect of membrane binding proteinA2(AA2)in the KB cell line of silent human luminal epithelial cancer cells on cell migration and invasion ability.Methods The expression of siRNA-AA2 cell-silent AA2 was transfected,the silencing effect was verified by the PCR experiment,and the ability of cell invasion was detected by Transwel.The ability of cell migration in each group was detected by scratch test.Results Compared with the control group,the expression of AA2 in si-AA2 group was significantly lower than that in the control group(P<0.05).Compared to the control group,the number of KB cells in si-AA2 group was significantly lower than that in the control group(P<0.05).Compared with the control group,the number of KB cells in si-AA2 group was significantly lower than that in the control group(P<0.05).After 24 h and 48 h of scratch cells,the healing area of si-AA2 group was significantly lower than that of the control group(P<0.05).Conclusion Interfering the expression of AA2 gene can inhibit the migration and invasion ability of KB cells in oral cell carcinoma.

关 键 词：膜联蛋白A2 口腔细胞癌 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]