Mn^2+和Co^2+胁迫对舞毒蛾幼虫营养和抗氧化力的影响  

Effects of Manganese Ion and Cobalt Ion Stress on Nutrition and Antioxidant Capacity of Gypsy Moth Larvae

作　　者：国嘉兴 曾健勇 石疆红 石中斌 张国财[1] 张杰[1] GUO Jia-xing;ZENG Jian-yong;SHI Jiang-hong;SHI Zhong-bing;ZHANG Guo-cai;ZHANG Jie(Northeast Forestry University,Harbin 150000,Heilongjiang,China)

机构地区：[1]东北林业大学,黑龙江哈尔滨150040

出　　处：《林业科学研究》2020年第1期131-135,共5页Forest Research

基　　金：中央高校基本科研业务费专项资金项目(2572017AA18);中央财政林业科技推广示范资金项目(JLT[2016]13);哈尔滨市应用技术研究与开发项目(2016RAXXJ035)。

摘　　要：[目的]为了明确舞毒蛾幼虫对Mn^2+和Co^2+胁迫的响应机制。[方法]采用0.40 mmol·g^-1 Mn^2+和0.83 mmol·g^-1 Co^2+的两种混合饲料分别喂食舞毒蛾4龄幼虫,测定了Mn^2+和Co^2+(LC30)对舞毒蛾4龄幼虫体质量、营养取食情况、营养成分含量及总抗氧化力。[结果]与对照组相比较,其取食量、虫粪量、体质量增长量均显著降低;Mn^2+处理组可溶性蛋白含量降低33.76%,碳水化合物含量降低57.56%,海藻糖含量降低64.05%,脂质含量降低27.77%;处理组可溶性蛋白含量降低47.46%,碳水化合物含量降低59.45%,海藻糖含量降低59.16%,脂质含量降低33.71%;Mn^2+处理组总抗氧化力增加28.25%,处理组总抗氧化力增加82.54%.表明Mn^2+和Co^2+对舞毒蛾幼虫取食量、虫粪量、体质量增长量、可溶性蛋白含量、碳水化合物含量、海藻糖含量、脂质含量均有显著的抑制作用,对其总抗氧化力有显著的促进作用。[结论]舞毒蛾幼虫是主要通过提高自身抗氧化力来应对重金属的胁迫,从而提高自身对重金属的抗性。[Objective]To determine the response mechanism of gypsy moth(Lymantria dispar)larvae to Mn^2+ and Co^2+ stress.[Method]The effects of 0.40 mmol Mn2^+·g^-1 and 0.83 mmol Co^2·g^-1 on body weight,nutrient intake,nutrient composition and total antioxidant capacity of the fourth instar larvae of gypsy moth were measured.[Result]Compared with the control group,the amount of food intake,feces and weight gain were significantly reduced.In the Mn2+treatment group,the soluble protein content reduced by 33.76%,carbohydrate content by 57.56%,trehalose content by 64.05%and lipid content by 27.77%.In Co^2+ treatment group,the soluble protein content reduced by 47.46%,carbohydrate content by 59.45%,trehalose content by 59.16%and lipid content by 33.71%.The total antioxidant capacity of the Mn2+treatment group increased by 28.25%,and that of the Co^2+ treatment group increased by 82.54%.These results showed that Mn^2+ and Co^2+ have significant inhibitory effects on the food intake,fecal content,weight growth,soluble protein content,carbohydrate content,trehalose content and lipid content of the larvae,and significantly promoted their total antioxidant capacity.[Conclusion]The larvae of gypsy moth respond to the stress of heavy metals mainly by improving the activity of their own antioxidant capacity,reducing sensitivity and improving resistance to heavy metals.

关 键 词：舞毒蛾 幼虫 锰离子 钴离子 营养取食 营养成分 总抗氧化力 

分 类 号：S763.42[农业科学—森林保护学]