猫尾草花叶病相关的新型马铃薯Y病毒属病毒  

Uraria crinita Mosaic Disease is Associated with a New Species of Potyvirus

作　　者：王韬远[1] 陶冶 陈长征[1] Wang TaoyuanTao Ye;Chen Changzheng(Wuhu Institute of Technology Landscape and Horticulture College,Wuhu,241003)

机构地区：[1]芜湖职业技术学院园林园艺学院

出　　处：《基因组学与应用生物学》2019年第11期5107-5112,共6页Genomics and Applied Biology

基　　金：安徽省自然科学重点项目(KJ2019A0981);芜湖职业技术学院校级自然科学重点项目(wzyzrzd201906);芜职院校级科技创新团队—生物资源开发与利用科技创新团队(Wzykj2018ZX01)共同资助

摘　　要：猫尾草(Uraria crinita)为豆科中草用药植物,亦称狗尾苔,在中国主要分布于福建、江西、广东、海南、广西、云南等地。江西地区发现叶片呈现花叶、黄化等病毒感染的病株。电子显微镜镜检,可观察到长度约800nm的长丝状病毒颗粒,患病细胞内可观察到风车状病毒内含体。机械接种于藜麦进行病毒单离,9~10 d后可观察叶脉处出现坏疽病斑。单离的病毒分离株回接猫尾草并未引起感染。萃取猫尾草患病叶组织总量RNA,配合Potyvirus属病毒简并式引物进行反转录聚合酶连锁反应(RT-PCR)所扩增出的cDNA片段,其序列与大豆花叶病毒(soybean mosaic virus,SMV)和watermelon mosaic virus(WMV)最为相似。解析病毒的全长度基因序列为9651个核苷酸,对应产生一个聚合蛋白。病毒基因结构与Potyvirus属病毒相同。病毒全长度基因核苷酸序列与WMV和SMV的相似度分别为67.6%和70.5%;外鞘蛋白基因核苷酸序列与WMV和SMV的相似度分别为74.0%和74.7%。本研究表明,猫尾草花叶病相关的病毒,应是Potyvirus属病毒的一个新病毒种。Uraria crinita is a medicinal plant of Leguminosae,also known as Dogtail moss.It is mainly distributed in Fujian,Jiangxi,Guangdong,Hainan,Guangxi,Yunnan and other places in China.Filamentous virus-like particles and pinwheel inclusion bodies induced by Potyvirus can be observed in the preparations of Uraria samples under electron microscopic examination.A virus isolate was obtained through mechanical inoculation onto Chenopodium quinoa.However,back inoculation of the virus to Uraria seedlings failed to show symptoms of infection.With Potyvirus degenerate primers and RT-PCR,expected cDNA fragments can be amplified and their nucleotide and amino acid sequences were most similar to that of soybean mosaic virus(SMV)and watermelon mosaic virus(WMV).Full-length genome which consists of 9651 nucleotides has been sequences.The genome encodes a polyprotein and its structure is identical to that of Potyvirus.The studied virus is most close to SMV and WMV among Potyvirus members based on their nucleotide sequences of full-length genome and coat protein(CP)gene.Its full-length genome shares 67.6%and 70.5%similarities to that of SMV and WMV,and its CP gene shares74.0%and 74.7%to that of SMV and WMV,respectively.It was shown that the virus associated to the mosaic disease of Uraria crinita is a tentative new member of Potyvirus.

关 键 词：马铃薯 Y病毒属 猫尾草 花叶病 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治]