单环刺螠(Urechis unicinctus)幼体肠道消化酶活性的测定方法  

Study on the determination methods of intestinal digestive enzymes activities in Echiuran WormUrechisunicinctus

作　　者：杨成林 林淦旻 常林瑞 刘学迁 刘峰[4] 赵彦翠[1] YANG Chen-lin;LIN Gan-min;CHANG Lin-rui;LIU Xue-qian;LIU Feng;ZHAO Yan-cui(School of Life Science,Ludong Universty,Yantai 264025,China;School of agriculture,Ludong Universty,Yantai 264025,China;Shandong Oriental Ocean Sci—Tech Co.,Ltd,Yantai,264100,China;Yantai Research Institute of China Agricultural University,Yantai 264670,China)

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]鲁东大学农学院,山东烟台264025 [3]山东省东方海洋科技股份有限公司,山东烟台264100 [4]中国农业大学烟台研究院,山东烟台264670

出　　处：《河北渔业》2020年第1期10-16,共7页

基　　金：烟台市科技计划项目(2017ZH056);山东省高等学校科技计划项目(J15LE53);山东省重点研发计划(2018GHY115028)

摘　　要：探究了单环刺螠(Urechis unicinctus)幼体(平均体重(1.468±0.222)g)肠道蛋白酶、淀粉酶、脂肪酶的测定方法、操作步骤、注意事项。蛋白酶测定采用Folin-酚法,其中建立的酪氨酸回归方程为y=15.487x-0.092 3(决定系数R2=0.999 2);淀粉酶测定采用淀粉-碘比色法,以0.08%的淀粉溶液为底物。脂肪酶测定采用聚乙烯醇橄榄油乳化液水解法(NaOH滴定法)。结果表明,单环刺螠幼体肠道蛋白酶活力、淀粉酶、脂肪酶活力分别为29.350±2.592、10.600±0.844、(5.266±0.224)U/mg prot。The determination methods,operation steps and precautions for operation methods of intestinal protease,amylase and lipase activity in juvenile Urechis unicinctus(average weight 1.468±0.222)g)were explored in detail.The intestinal protease,amylase and lipase activity in juvenile Urechis unicinctus was respectively determined by method of Folin—phenol,starch—iodine colorimetry and hydrolysis of polyvinyl alcohol emulsion of olive oil(titration of NaOH).In order to determine the intestinal protease activity assay in juvenile Urechis unicinctus the established regression equation of tyrosine was y=15.487 x-0.0923(R2=0.9992).The intestinal protease activity in juvenile Urechis unicinctus was(29.350±2.592)U/mg prot.The intestinal amylase activity was(10.600±0.844)U/mg prot with 0.08% starch solution as substrate.The intestinal lipase activity was(5.266±0.224)U/mg prot.

关 键 词：单环刺螠(Urechis unicinctus) 肠道 蛋白酶 淀粉酶 脂肪酶 测定方法 

分 类 号：S917.4[农业科学—水产科学]