食品中硒的总量及化学形态分析研究进展  

Total content determination and chemical speciation analysis of selenium in food: A review

作　　者：赵谋明[1,2] 郑泽洋 刘小玲[1,3] ZHAO Mou-ming;ZHENG Ze-yang;LIU Xiao-ling(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;College of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China;Guangxi Buffalo Milk Engineering and Technology Center,Nanning 530004,China)

机构地区：[1]广西大学轻工与食品工程学院,南宁530004 [2]华南理工大学食品科学与工程学院,广州510640 [3]广西水牛乳工程技术研究中心,南宁530004

出　　处：《南方农业学报》2019年第12期2787-2796,共10页Journal of Southern Agriculture

基　　金：广西创新驱动发展专项(桂科AA17202010-3,桂科AA17204075)

摘　　要：硒是生物体内必需的微量元素,其毒性和生物活性与化学形态相关,文章结合国内外研究情况从食品中总硒的测定和硒化学形态分析两个方面进行综述。在总硒测定方法中,采用超声辅助提取总硒,并以原子吸收作为检测器时,方法的灵敏度高,检测限低;而在硒化学形态分析中,固相萃取是效率高、准确性高、应用范围广的提取方法,结合气相色谱-质谱联用法(GC-MS)进行测定硒化物含量时,方法的检测限较低。由于其他形态的硒含量低或结构复杂,难以从基质中分离鉴定,目前主要关注5种形态的硒[Se(VI)、Se(IV)、SeMet、SeCys和Se-MeSeCys],且存在总硒测定和硒形态分析的过程均耗时较长等问题,因此今后需关注更多未知形态的硒,以便更好地利用硒,并开发绿色快速提取、分离及检测一体化的自动化检测技术。Selenium is an essential trace element in organisms,whose toxicity and biological activity are related to its morphology. This article reviewed the two aspects of the determination of total selenium and selenium chemical speciation analysis in food from home and abroad. In case of total selenium determination,the determination method with high sensitivity and low detection limit is consisted of ultrasound-assisted extraction as a extraction way and the atomic absorption as a detector. While in case of selenium speciation analysis,solid-phase extraction is an efficient,accurate and popular way to extraction the selenium speciation. After that,used the gas cromatography-mass spectrometry(GC-MS)as the detector would obtain the lower detection limit in all methods. Due to the low content and complex structure of selenium compounds,it is difficult to separate and identify them from the matrix. And five forms of selenium are mainly concerned now[Se(VI),Se(IV),SeMet,SeCys and Se-MeSeCys]. Moreover,it has spending a long time in the process of total selenium determination and selenium form analysis. To resolve these problems,firstly,the researchers should pay attention to find more unknown selenium compounds in order to make better use of them;secondly,researchers should develop an integrated detection technology of green and fast extraction,separation and detection.

关 键 词：硒 含量 形态分析 预处理 检测技术 

分 类 号：S-03[农业科学] TS201.4[轻工技术与工程—食品科学]