超高效液相色谱柱后衍生装置测定饮用水中的草甘膦  

Determination of Glyphosate in Drinking Water with Ultra-High Performance Liquid Chromatography post-Column Derivative Device

作　　者：陈颖[1] 王珍妮 宋月[1] 白欣[1] CHEN Ying;WANG Zhen-ni;SONG Yue;BAI Xin(Physical and Chemical Testing Institute of Dalian Municipal Center for Disease Control and Prevention,Dalian 116021,China)

机构地区：[1]大连市疾病预防控制中心理化技术检验所

出　　处：《中国医药指南》2020年第1期2-3,共2页Guide of China Medicine

摘　　要：目的建立饮用水中草甘膦的超高效液相色谱柱后衍生方法。方法在700 m L水样中分批次加入盐酸进行浓缩,经阳离子交换柱后与衍生剂邻苯二醛、β-巯基乙醇反应,利用荧光检测器检测。以保留时间定性,峰面积定量。结果草甘膦在5~1000 ng范围内呈线性相关。方法精密度良好(RSD<5%,n=6),回收率在80%~100%,检出限为0.1μg/m L(S/N=3)。结论本方法特异性强,灵敏度高,适合于生活饮用水中的草甘膦检测。Objective To develop a Post-Column derivatization of ultra-high performance liquid chromatography for detection of glyphosate in drinking water. Methods 700 mL of water samples were concentrated by addition of hydrochloric acid, reacted with the derivatives, O-phthalaldehyde and β-mercaptoethanol after the cation exchange column, and determined by fluorescence detector. The target compound was qualified by retention time and quantified by peak area. Results A linear correlation was achieved with the glyphosate in the range of 5-1000 ng. The method had good precision(RSD<5%, n=6), and the recovery of glyphosate was 80%-100%, with the detection limit of 0.1 μg/mL(S/N=3). Conclusion This work is specific and sensitive, which is suitable for glyphosate detection in drinking water.

关 键 词：草甘膦 超高效液相色谱柱后衍生装置 饮用水 

分 类 号：R-33[医药卫生]