姜黄素对骨肉瘤细胞增殖、凋亡的影响及作用机制研究  

Effect and Mechanism of Cur cumin on Proliferation and Apoptosis ofOsteosarcoma Cells

作　　者：吴峻[1] WU Jun

机构地区：[1]宝鸡职业技术学院,陕西宝鸡721000

出　　处：《西部微创骨科》2018年第4期5-10,共6页Western Journal of Minimally Invasive Orthopedics

基　　金：陕西省科技攻关计划项目(编号:2015K09-16)。

摘　　要：目的:探讨姜黄素对骨肉瘤细胞增殖、凋亡的影响及作用机制。方法:选用骨肉瘤MG-63细胞株,分别采用不同浓度的姜黄素进行处理。采用MTT比色法检测骨肉瘤MG-63细胞增殖抑制率,釆用流式细胞仪检测骨肉瘤MG-63细胞凋亡情况,采用Western Blot法检测骨肉瘤MG-63细胞凋亡相关蛋白表达水平。结果:1)干预12h、24h、48h后,与对照组比较,姜黄素各浓度组骨肉瘤MG-63细胞增殖抑制率较高,且姜黄素5μmoL/L组<10μmoL/L组<15μmoL/L组及20μmoL/L组,差异有统计学意义(P<0.05)。2)干预48h后,与对照组比较,姜黄素各浓度组骨肉瘤MG-63细胞凋亡率较高,且姜黄素5μmol/L组<10μmol/L组<15μmol/L组,差异有统计学意义(P<0.05). 3)与对照组比较,黄素各浓度组骨肉瘤MG-63细胞P53、B细胞淋巴瘤/白血病-2(Bcl-2)、Caspase-9前体(proCaspase-9)蛋白表达水平较低,且姜黄素5μmoL/L组>10μmoL/L组>15μmoL/L组,差异有统计学意义(P<0.05). 4)与对照组比较,姜黄素各浓度组骨肉瘤MG-63细胞Bcl相关蛋白(Bax)、细胞色素C(Cyt-C)、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)蛋白表达水平较高,且姜黄素5μmoL/L组<10μmoL/L组<15μmoL/L组,差异有统计学意义(P<0.05)。结论:姜黄素可剂量依赖性的抑制骨肉瘤MG-63细胞增殖,并促进其凋亡,其作用可能与下调P53、Bcl-2、proCaspase-9蛋白表达,上调Bax、Cyt-C、Caspase-3蛋白表达,激活线粒体凋亡途径有关。Objective:To analysis the effects and mechanism of anticancer drug curcumin on proliferation and apoptosis of osteosarcoma cells.Methods:Osteosarcoma MG-63 cells were selected and treated with different concentrations of curcumin.The osteosarcoma MG-63 cells proliferation inhibition rate was detected by MTT colorimetric method,apoptosis of osteosarcoma MG-63 cells was detected by flow cytometry,the expression levels of apoptosis related protein were detected by Western Blot method in osteosarcoma MG-63 cells.Results:1)After the intervention of 12h,24h and 48h,compared with control group,the proliferation inhibition rate of osteosarcoma MG-63 cells were higher in each concentration of curcumin group,and curcumin 5μmoL/L group<10μmol/L group<15μmoL/L group and 20μmol/L group,the differences were statistically significant(P<0.05).2)Af ter the intervention of 48 h,compared with control group,the apoptosis rate of osteosarcoma MG-63 cells were higher in each concentration of curcumin group,and curcumin 5μmoL/L group<10μmoL/L group<15μmol/L group,the differences were statistically significant(P<0.05).3)Compared with control group,the expression of P53,B cell lymphoma/leukemia-2(Bcl-2),Caspase-9 precursor(proCaspase-9)protein in osteosarcoma MG-63 cells were lower in each concentration of curcumin group,and curcumin 5μmol/L group>10μmoL/L group>15 nmol/L group,the differences were statistically significant(P<0.05).4)Compared with control group,the expression of Bel related proteinffiax),cytochrome C(Cyt-O,aspartate specific cysteine protease-3(Caspase-3)protein in osteosarcoma MG-63 cells were higher in each concentration of curcumin group,and curcumin 5μmol/L group<10μmol/L group<15μmol/L group,the differences were statistically significant(P<0.05).Conclusions:Anticancer drug curcumin can inhibit the proliferation and promote apoptosis of osteosarcoma MG-63 cells in a dose-dependent manner,the effects may be related to down-regulation of P53,Bcl-2,proCaspase-9 protein expression,up-regulation of Bax,Cyt-C,C

关 键 词：姜黄素 骨肉瘤细胞 细胞增殖 细胞凋亡 机制 

分 类 号：R73[医药卫生—肿瘤]