基于丝氨酸/苏氨酸激酶信号观察芬太尼对鼻咽癌细胞增殖侵袭的作用机制  

Mechanism of fentanyl on proliferation and invasion of nasopharyngeal carcinoma cells based on serine/threonine kinase signal

作　　者：张宇帆[1] 郭俊[1] 乐新会[1] ZHANG Yufan;GUO Jun;LE Xinhui(Department of Anesthesiology,Jinhua Central Hospital,Jinhua,Zhejiang,321000,China)

机构地区：[1]金华市中心医院麻醉科

出　　处：《中国耳鼻咽喉头颈外科》2019年第12期674-677,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的基于丝氨酸/苏氨酸激酶(RhoA/Rho)信号通路观察芬太尼(简称芬太尼)对鼻咽癌细胞增殖侵袭的影响。方法传代培养高侵袭性鼻咽癌细胞系CNE2,分为空白对照组(加入无血清培养基)、抑制剂组(加入RhoA激酶抑制剂C3转化酶)、芬太尼组(加入药物芬太尼)。MTT实验、划痕实验及Transwell检测细胞增殖、迁移和侵袭能力,采用qRT-PCR法、Western blot法检测 RhoA、Rho的mRNA和蛋白表达。采用Western blot法检测RhoA/Rho激活后的下游效应产物MYPT1蛋白。结果与空白对照组比较,芬太尼组与抑制剂组细胞克隆数、细胞增殖率、细胞迁移率均显著降低(t=24.947、22.329、5.361、4.789、9.262、8.968,P均<0.05);抑制剂组与芬太尼组RhoA以及Rho的mRNA转录水平显著低于空白对照组(t=7.712、8.261、7.983、7.905,P均<0.05);与空白对照组相比,芬太尼组与抑制剂组的RhoA、Rho以及MYPT1蛋白表达明显降低(t=2.661、3.105、3.862、3.429、4.656、4.648,P均<0.05)。结论芬太尼可抑制鼻咽癌细胞的增殖和侵袭,该作用可能与其抑制Roha/Rho激酶信号密切相关。OBJECTIVE To observe the effect of fentanyl on proliferation and invasion of nasopharyngeal carcinoma cells based on rho-associated coiled-coil-forming kinases (RhoA/Rho).METHODS The highly invasive nasopharyngeal carcinoma (NPC) cell line CNE2 was subcultured and divided into blank control group (added serate-free medium),inhibitor group (added RhoA kinase inhibitor C3 invertase) and fentanyl group (added drug fentanyl).MTT assay,scratch assay and Transwell assay were used to detect the proliferation,migration and invasion ability of cells.MRNA and protein expressions of RhoA and Rho were detected by qRT-PCR and Western blot.The downstream effector protein MYPT1 after RhoA/Rho activation was detected by Western blot.RESULTS Compared with the blank control group,the cell clone number,cell proliferation rate and cell mobility of fentanyl group and inhibitor group were significantly reduced (t=24.947,22.329,5.361,4.789,9.262,8.968,P<0.05).The mRNA transcription levels of RhoA and Rho in inhibitor group and fentanyl group were significantly lower than that in blank control group (t=7.712,8.261,7.983,7.905,P<0.05).Compared with the blank control group,the expressions of RhoA,Rho and MYPT1 protein in fentanyl group and inhibitor group were significantly decreased(t=2.661,3.105,3.862,3.429,4.656,4.648,P<0.05).CONCLUSION Fentanyl can inhibit the proliferation and invasion of nngeal carcinoma cells,which may be closely related to its inhibition of Roha/Rho kinase signal.

关 键 词：鼻咽肿瘤 芬太尼 细胞增殖 细胞侵袭 丝氨酸/苏氨酸激酶信号通路 

分 类 号：R739.63[医药卫生—肿瘤]