机构地区:[1]湖北中医药高等专科学校
出 处:《河北医学》2020年第2期329-333,共5页Hebei Medicine
基 金:湖北省卫生和计划生育委员会科研项目,(编号:WJ2017MB086)
摘 要:目的:研究胡桃醌对口腔鳞癌Tca8113细胞的影响,并检测其对CDK4表达的调控作用,探讨其可能的作用机制。方法:将口腔鳞癌Tca8113细胞分为4组,分别是对照组(0μmoL/L)、胡桃醌低剂量组(25μmoL/L)、胡桃醌中剂量组(50μmoL/L)、胡桃醌高剂量组(100μmoL/L),倒置显微镜观察不同浓度组细胞形态学变化;采用MTT法检测胡桃醌对Tca8113细胞增殖抑制作用;流式细胞仪分析胡桃醌对Tca8113细胞周期分布的影响;运用免疫细胞化学法分析各浓度组Tca8113细胞中CDK4的表达情况。结果:胡桃醌作用细胞24h后,细胞数量减少,细胞质变粗糙,细胞变得更小和圆润,胡桃醌浓度越高,变化越明显。MTT结果显示,胡桃醌低、中、高剂量组Tca8113细胞OD值均低于对照组(P<0.05),胡桃醌中、高剂量组Tca8113细胞OD值低于胡桃醌低剂量组(P<0.05);胡桃醌高、中、低剂量组细胞凋亡率明显高于对照组(P<0.05),胡桃醌高、中剂量组细胞凋亡率高于胡桃醌低剂量组(P<0.05),胡桃醌高剂量组高于胡桃醌中剂量组(P<0.05);胡桃醌高、中、低剂量组CDK4阳性率明显低于对照组(P<0.05),胡桃醌高、中剂量组CDK4阳性率低于胡桃醌低剂量组(P<0.05),胡桃醌高剂量组CDK4阳性率低于胡桃醌中剂量组(P<0.05)。结论:胡桃醌可抑制口腔鳞癌Tca8113细胞的增殖活性,诱导细胞凋亡,下调CDK4的表达,抑制肿瘤的侵袭。Objective:To investigate effect of juglone on proliferation and apoptosis of oral squamous cell carcinoma Tca8113 cells by regulating CDK4 expression.Methods:Oral squamous cell carcinoma Tca8113 cells were divided into 4 groups:control group,low-dose,middle-dose,high-dose juglone groups(0μmoL/L,25μmoL/L,50μmoL/L,100μmoL/L).The morphological changes of cells in different concentration groups were observed by inverted microscope.The inhibitory effect of juglone on the proliferation of Tca8113 cells was determined by MTT assay.The effect of walnut juglone on Tca8113 cell cycle distribution was analyzed by flow cytometry.The expression of CDK4 in Tca8113 cells was analyzed by immunocytochemistry.Results:24 hours after the action of walnut quinone,the number of cells was decreased,the cytoplasm became rough,the cells became smaller and rounder,and the higher the concentration of walnut quinone,the more obvious the change was.MTT results showed that OD values of Tca8113 cells in low,medium and high dose groups were lower than those in control group(P<0.05),OD values of Tca8113 cells in medium and high dose groups were lower than those in low dose group(P<0.05),apoptotic rates in high,medium and low dose groups were significantly higher than those in control group(P<0.05),and apoptotic rates in high,medium and low dose groups were higher than those in low dose group(P<0.05).The positive rate of CDK4 in high-dose group was higher than that in medium-dose group(P<0.05);the positive rate of CDK4 in high,middle and low-dose group was lower than that in control group(P<0.05);the positive rate of CDK4 in high and middle-dose group was lower than that in low-dose group(P<0.05);and the positive rate of CDK4 in high-dose group was lower than that in middle-dose group(P<0.05).Conclusion:Juglone can inhibit the proliferation of oral squamous cell carcinoma Tca8113 cells,induce apoptosis,down-regulate the expression of CDK4 protein,and inhibit the invasion of tumors.
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