转染沉默IGFBP-2基因抑制食管癌细胞系TE-1增殖、转移的影响  

The Effect of Transfecting and Silencing Gene IGFBP-2 on Inhibition of Proliferation and Metastasis of Esophageal Carcinoma Cell Line TE-1

作　　者：张倬 熊飞 李雪曼 陈天佑 Zhang Zhuo;Xiong Fei;Li Xueman;Chen Tianyou(Department of Thoracic Surgery,The Third Hospital of Wuhan,Wuhan 430000,China)

机构地区：[1]武汉市第三医院胸外科

出　　处：《甘肃科学学报》2020年第1期32-37,共6页Journal of Gansu Sciences

基　　金：武汉市卫计委基金资助项目(WX17D08)

摘　　要：研究转染沉默IGFBP-2抑制食管癌细胞系TE-1增殖、转移的影响。通过构建好的IGFBP-2的SiRNA绿色荧光载体转染到人食管癌细胞系TE-1中,于荧光显微镜下观测转染效率,通过Western-blot证实基因沉默的效果,并通过CCK8,Transwell和流式来分别评估IGFBP-2-SiRNA对细胞增殖,侵袭和凋亡的影响。结果显示:Western-blot法证实IGFBP-2能有效地被SiRNA沉默,而且IGFBP-2-SiRNA能抑制食管癌细胞系TE-1的增殖\,侵袭和诱导凋亡。实验结果表明IGFBP-2在食管癌的发病机理中可能有着重要的作用。IGFBP-2也许能成为食管癌治疗的一个靶向基因。This paper is to study the effect of transfecting and silencing gene IGFBP-2 on inhibition of proliferation and metastasis of esophageal carcinoma cell line TE-1.By transfecting IGFBP-2 to esophageal carcinoma cell line TE-1 through the ready SiRNA green fluorescent carrier,the transfection efficiency was observed under a fluorescence microscope,the effect of gene silencing was verified by means of western-blot,and the effect of IGFBP-2-SiRNA on cell proliferation,invasion and apoptosis was evaluated respectively with CCK8,Transwell and flow cytometry.The western-blot method confirmed that IGFBP-2 could be effectively silenced by SiRNA,and that IGFBP-2-SiRNA could inhibit the proliferation,invasion and induced apoptosis of esophageal carcinoma cell line TE-1.The experimental results show that IGFBP-2 may play an important role in the etiopathogenesis of esophageal cancer.IGFBP-2 may be a target gene for esophageal cancer treatment.

关 键 词：IGFBP-2 食管癌 SIRNA TE-1 

分 类 号：R735.1[医药卫生—肿瘤]