lncRNA PVT1沉默对卵巢癌SKOV3细胞增殖、迁移及STAT3通路的影响  

Effects of lncRNA PVT1 silencing on proliferation,migration and STAT3 pathway of ovarian cancer SKOV3 cells

作　　者：赵书君[1] 樊素珍 牛志军 石岩玉 Zhao Shujun;Fan Suzhen;Niu Zhijun;Shi Yanyu(Department of Gynecology,the Third Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China.)

机构地区：[1]郑州大学第三附属医院妇瘤科

出　　处：《现代肿瘤医学》2020年第4期534-538,共5页Journal of Modern Oncology

基　　金：郑州市技术研究与开发经费支持项目(编号:153PKJGG165)

摘　　要：目的:研究沉默变异性浆细胞瘤异位1(plasmacytoma heterotopic 1,PVT1)基因对卵巢癌SKOV3细胞增殖、迁移、信号转导及转录活化因子(STATs)3通路的影响。方法:体外培养卵巢癌SKOV3细胞株,设计PVT1 siRNA序列,转染细胞,分为siRNA组、阴性对照组(NC)、空白对照组(BC),利用qRT-PCR检测各组细胞PVT1相对表达量,利用MTT法检测细胞增殖情况,利用划痕实验检测细胞迁移能力,利用Transwell实验检测细胞侵袭能力,利用WB法检测STAT3通路相关蛋白表达情况。结果:转染后,siRNA3组细胞中PVT1表达水平较BC组与NC组显著降低(P<0.05);转染后,与BC组与NC组比较,siRNA3组细胞增殖率较BC组与NC组显著降低(P<0.05),迁移、侵袭细胞数显著降低(P<0.05),p-STAT3蛋白,MMP2、MMP9、CD44、PCNA蛋白表达水平显著降低(P<0.05)。结论:沉默PVT1可能抑制卵巢癌SKOV3细胞增殖、迁移与侵袭,其具体机制可能与STAT3信号通路有关。To study the effects of silencing variant plasmacytoma heterotopic 1(PVT1)gene on proliferation,migration,signal transduction and activation factor(STATs)3 pathway of ovarian cancer SKOV3 cells.Methods:SKOV3 cell line of ovarian cancer was cultured in vitro.The sequence of PVT1 siRNA cell was designed,cells were transfected.The study in cluded siRNA group,negative control group(NC)and blank control group(BC).The relative expression of PVT1 was detected by qRT-PCR.MTT assay was used to detect cell proliferation.Scratch test was used to detect cell migration ability.Transwell assay was used to detect cell invasiveness.The expression of STAT3 pathway-related protein was detected by WB method.Results:After transfection,the expression of PVT1 in cells in siRNA3 group was significantly lower than that in BC and NC groups(P<0.05).After transfection,compared with BC group and NC group,the cell proliferation rate of siRNA3 group was significantly lower than that of BC group and NC group(P<0.05),the numbers of migrating and invading cells decreased significantly(P<0.05),the expression levels of p-STAT3 protein,MMP2,MMP9,CD44 and PCNA were significantly decreased(P<0.05).Conclusion:Silencing PVT1 may inhibit the proliferation,migration and invasion of ovarian cancer SKOV3 cells,and its specific mechanism may be related to STAT3 signaling pathway.

关 键 词：卵巢癌 变异性浆细胞瘤异位1 信号转导及转录活化因子3 细胞增殖 细胞迁移 

分 类 号：R737.1[医药卫生—肿瘤]