miR-147对胶质瘤U87细胞增殖、凋亡和侵袭的影响  

Study on the correlation between miR-147 and biological activity of U87 cells in glioma cells

作　　者：许小斌 武伟男 马宁[2] 连世忠[2] Xu Xiaobin;Wu Weinan;Ma Ning;Lian Shizhong(The First Clinical Medical College,Shanxi Medical University,Shanxi Taiyuan 030001,China;Department of Neurosurgery,the First Hospital of Shanxi Medical University,Shanxi Taiyuan 030001,China)

机构地区：[1]山西医科大学第一临床医学院,山西太原030001 [2]山西医科大学第一医院神经外科,山西太原03000

出　　处：《现代肿瘤医学》2020年第3期360-363,共4页Journal of Modern Oncology

基　　金：山西省基础研究计划项目(自然)(编号:2015011095)

摘　　要：目的:比较胶质瘤患者胶质瘤组织和瘤旁组织中miR-147表达水平的差异性,进一步分析miR-147与胶质瘤细胞生物学活性的相关性。方法:通过实时荧光定量PCR检测65例胶质瘤患者瘤组织和瘤旁组织中miR-147的表达水平;采用Lipofectamine 2000转染法转染胶质瘤细胞,分为miR-147 mimics组和NC组,检测两组转染效率;进一步通过CCK-8实验、流式细胞实验和Transwell实验比较两组细胞生物活性。结果:胶质瘤组织中miR-147表达水平显著低于瘤旁组织;与NC组比较,miR-147 mimics组细胞不论是增殖能力还是侵袭能力都明显降低,但是,miR-147 mimics组的细胞凋亡率升高。结论:胶质瘤组织中存在miR-147低表达的现象。miR-147在胶质瘤细胞中表达升高能够抑制细胞的增殖能力、降低其侵袭能力,并促使更多的细胞发生晚期凋亡。miR-147可能在胶质瘤中扮演着抑癌基因的重要角色。Objective:To compare the difference of miR-147 expression between glioma tissues and adjacent tissues,and to further analyze the correlation between miR-147 and biological activity of glioma cells.Methods:The expression of miR-147 in 65 glioma tissues and adjacent tissues was detected by real-time fluorescence quantitative PCR,and the glioma cells were transfected into miR-147 mimics group and NC group by Lipofectamine 2000 method,and the transfection efficiency of the two groups was detected.Furthermore,CCK-8 assay,flow cytometry test and Transwell assay were used to compare the cell biological activity between the two groups.Results:The expression level of miR-147 in glioma tissue was significantly lower than that in paratumor tissue,and compared with NC group,the proliferation and invasion ability of miR-147 mimics group were decreased,but the apoptosis rate was increased.Conclusion:There is a low expression of miR-147 in glioma tissues.Overexpression of miR-147 can inhibit the proliferation and invasion of glioma cells and induce apoptosis of glioma cells,which plays an anti-cancer role.

关 键 词：miR-147 胶质瘤 增殖 凋亡 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]