MicroRNA-192对结肠癌RKO细胞增殖、迁移和侵袭能力的影响及机制研究  

The influence of microRNA-192 on proliferation,migration and invasion of RKO colon cancer cells and mechanism

作　　者：范盼红 赵文月 贾琳娇[3] 刘秋雨[1] 赵跃武[1] Fan Panhong;Zhao Wenyue;Jia Linjiao;Liu Qiuyu;Zhao Yuewu(Pathology Department,Henan Provincial People's Hospital,Henan Zhengzhou 450003,China;Endocrinology Department,Dandong Central Hospital,Liaoning Dandong 118010,China;Breast Surgery,Henan Provincial People's Hospital,Henan Zhengzhou 450003,China)

机构地区：[1]河南省人民医院病理科,河南郑州450003 [2]丹东市中心医院内分泌科,辽宁丹东118010 [3]河南省人民医院乳腺外科,河南郑州450003

出　　处：《现代肿瘤医学》2020年第3期374-379,共6页Journal of Modern Oncology

基　　金：河南省医学科技攻关计划项目(编号:201602248)

摘　　要：目的:探讨microRNA-192(miR-192)对于高侵袭性结肠癌RKO细胞的增殖、迁移和侵袭能力的影响。方法:将miR-192 mimics(miR-192)通过脂质体转染至RKO细胞,应用qRT-PCR检测细胞中miR-192表达情况,采用CCK-8实验和集落形成实验研究miR-192对结肠癌RKO细胞增殖的影响,采用细胞划痕实验和Transwell小室模型检测miR-192对结肠癌细胞迁移和侵袭能力的影响,应用Western blot检测miR-192对结肠癌RKO细胞内上皮性钙黏附蛋白(E-cadherin,E-cad)和血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达的影响。结果:CCK-8实验和集落形成实验结果显示:转染了miR-192的结肠癌RKO细胞,癌细胞增殖显著受到抑制。细胞划痕实验、迁移实验和细胞侵袭实验结果显示:RKO细胞转染miR-192后,迁移和侵袭的细胞数量显著减少,差异有统计学意义(P<0.05)。Western blot结果显示:结肠癌细胞转染miR-192后可升高E-cad蛋白的表达,并且降低VEGF蛋白的表达。结论:转染miR-192可抑制结肠癌RKO细胞增殖,降低其迁移和侵袭能力,其可能的机制是升高结肠癌RKO细胞E-cad的表达,并且降低VEGF的表达。miR-192可能作为肿瘤诊断及治疗的新靶点。Objective:To investigate the influence of microRNA-192(miR-192)on proliferation,migration and invasion of RKO colon cancer cell and the potential mechanism.Methods:miR-192 mimics(miR-192)was transfected into human colon cancer cell line RKO using Oligofectamine.qRT-PCR was used to measure the expression level of miR-192.The proliferation of miR-192 was measured by CCK-8 and colony formation assays.Wound-healing,Transwell chamber were used to evaluate the migration and invasion ability.Western blot detected the effect of miR-192 on E-cad and VEGF expression.Results:The CCK-8 and colony formation assays showed that miR-192 can inhibit the proliferation of RKO cells.The number of migrating cells reduced sharply by cell counting of Wound-healing and Transwell assays.The invasion of RKO cells was inhibited compared with the Control group and NC group(P<0.05).Western blot assay revealed that E-cad increased and VEGF decreased significantly by miR-192.Conclusion:miR-192 could effectively inhibit the growth and invasion and migration ability of colon cancer cells.miR-192 increased E-cad and decreased VEGF expression may play an important role in the process.The findings of this study suggest that miR-192 may have a unique potential as a novel biomarker candidate for tumor diagnosis and therapy.

关 键 词：miR-192 结肠癌RKO细胞 上皮性钙黏附蛋白 血管内皮生长因子 

分 类 号：R735.35[医药卫生—肿瘤]