蛇床子素对胃BGC-823细胞的体外放疗增敏作用的实验研究  

Enhancement of radiation effects by Osthole on human gastric BGC-823 cell line in vitro

作　　者：毛佳蕾 杨华[1] 陆琼[1] 金丽娟 Mao Jialei;Yang Hua;Lu Qiong;Jin Lijuan(Department of Oncology,Kunshan Hospital of Traditional Chinese Medicine,Jiangsu Kunshan 215300,China)

机构地区：[1]昆山市中医医院肿瘤内科

出　　处：《现代肿瘤医学》2020年第3期379-382,共4页Journal of Modern Oncology

基　　金：昆山市科技计划项目(编号:KS1558)

摘　　要：目的:研究中药单体蛇床子素(Ost)对胃BGC-823细胞的体外放疗增敏作用。方法:采用MTT法检测单药及联合放疗对BGC-823细胞的作用;细胞克隆形成实验检测Ost对BGC-823细胞的放疗增敏作用;流式细胞术分析Ost及放疗对BGC-823细胞的细胞周期及细胞凋亡的影响。结果:Ost对BGC-823细胞具有增殖抑制作用;低细胞毒剂量的Ost(15μg/ml)对BGC-823细胞具有放疗增敏作用,经单击多靶模型拟合后,Ost联合放疗组细胞存活曲线较单纯放疗组左移,D0、Dq值变小,放疗增敏比SER=1.64。结论:低细胞毒剂量的Ost(15μg/ml)对BGC-823细胞具有放疗增敏作用,其机制可能与增加放疗诱导的细胞凋亡,引起放疗敏感时相G1期细胞阻滞,减少放疗相对抗拒时相S期细胞比例相关。Objective:To study the enhancement of radiation effects by Osthole on gastric BGC-823 cells in vitro.Methods:MTT assay detected the effects of single drug and combined radiotherapy on BGC-823 cells.Cell clone formation assay detected the enhancement of radiation effects by Ost on BGC-823 cells.Flow cytometry analyzed the effects of Ost and radiotherapy on cell cycle and apoptosis in BGC-823 cells.Results:Ost could inhibit the proliferation of BGC-823 cells.Low cytotoxic dose of Ost(15μg/ml)had radiotherapy sensitization effect on BGC-823 cells.After single-click multi-target model fitting,the cell surviving fraction of Ost combined radiotherapy group was shifted to the left,compared with the radiotherapy group,and D 0 and D q values were reduced,and the radiotherapy sensitization ratio SER=1.64.Conclusion:Low cytotoxic dose of Ost(15μg/ml)has radiotherapy sensitization effect on BGC-823 cells.The mechanism may be related to increasing radiation-induced apoptosis,causing cell block in phase G 1 when there is radiotherapy sensitization,and reducing the proportion of cells in phase S when there is radiotherapy resistance.

关 键 词：蛇床子素(Ost) 细胞克隆形成实验 细胞周期 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]