吉西他滨联合顺铂动、静脉介入化疗对肺癌移植瘤大鼠肿瘤生长抑制作用的比较  

Inhibitory effect of gemcitabine combined with cisplatin on tumor in rats with lung cancer

作　　者：李晓东 刘庆祥[2] Li Xiaodong;Liu Qingxiang(Department of Radiology,the Third Hospital of Chengde,Hebei Chengde 067000,China;Department of Radiology,Chengde Central Hospital,Hebei Chengde 067000,China)

机构地区：[1]承德市第三医院放射科,河北承德067000 [2]承德市中心医院放射科,河北承德067000

出　　处：《现代肿瘤医学》2020年第3期387-391,共5页Journal of Modern Oncology

摘　　要：目的:比较吉西他滨联合顺铂动、静脉介入化疗对肺癌移植瘤大鼠肿瘤生长抑制作用的差异,探讨最佳给药途径。方法:将40只大鼠随机分为四组,每组10只。根据给药方案的不同,分为空白对照组、生理盐水(NS)对照组、静脉化疗组与动脉化疗组。动态检测四组大鼠的肿瘤体积、瘤重抑瘤率变化。处死大鼠后,利用Western-blot法检测瘤体Bcl-2、Caspase-3蛋白表达量,免疫组化检测Ki-67表达。结果:随着治疗时间的延长,空白对照组与NS对照组的大鼠肿瘤体积逐渐增大,静脉化疗组与动脉化疗组肿瘤体积逐渐缩小,且随着给药时间延长,抑制效果优势愈加明显。动脉化疗组抑瘤率(57.6%)比静脉化疗组(42.4%)更高,差异具有统计学意义(P<0.05)。Western-blot检测发现,与空白对照组、NS对照组相比,静脉化疗组与动脉化疗组大鼠均出现Caspase-3蛋白表达量升高,Bcl-2蛋白表达量下降。两两比较,动脉化疗组大鼠的Bcl-2蛋白表达量最低,Caspase-3蛋白表达量最高。静脉化疗组与动脉化疗组均有凋亡细胞出现,但动脉化疗组凋亡细胞比例更高。四组大鼠的瘤体均有Ki-67阳性表达,空白对照组与NS对照组Ki-67增殖系数最高,静脉化疗组次之,动脉化疗组Ki-67的增殖系数最低。结论:吉西他滨联合顺铂经动脉灌注的比静脉化疗的抑瘤效果更好。Objective:To compare the inhibitory effects of gemcitabine combined with cisplatin on tumor growth in rats with lung cancer.Methods:40 rats were randomly divided into blank control group,normal saline(NS)group,intravenous chemotherapy group and arterial chemotherapy group.The changes of tumor volume,tumor inhibition rate and the expression of Bcl-2 and Caspase-3 protein were detected.Results:With the prolongation of treatment time,the tumor volume of the blank control group and NS group increased gradually,the tumor volume of intravenous chemotherapy group and arterial chemotherapy group decreased gradually,and with the prolongation of administration time,the inhibition effect advantage became more obvious.The tumor inhibition rate(57.6%)in arterial chemotherapy group was higher than intravenous chemotherapy group(42.4%).Western-blot showed that the expression of Caspase-3 protein increased and the expression of Bcl-2 protein decreased in intravenous chemotherapy group and arterial chemotherapy group compared with the blank control group and NS group.The expression of Bcl-2 protein was lowest and Caspase-3 protein was highest in arterial chemotherapy group.There were apoptotic cells in intravenous chemotherapy group and arterial chemotherapy group,and the apoptotic cells ratio was higher in arterial chemotherapy group.Ki-67 positive expression was found in all the tumors of the four groups.The proliferation coefficient of Ki-67 in the blank control group and NS group was higher than that in the intravenous chemotherapy group,and the proliferation coefficient of Ki-67 in the arterial chemotherapy group was the lowest.Conclusion:Gemcitabine combined with cisplatin in arterial chemotherapy group is more effective in inhibiting tumor growth than intravenous chemotherapy.

关 键 词：多西他赛 顺铂 介入治疗 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]