KLF4在弥漫性大B细胞淋巴瘤组织中的表达及其作用机制研究  

Expression of KLF4 in diffuse large B-cell lymphoma and its mechanism

作　　者：林志美[1] 李建[1,2] 饶进[1] 邹忠晴 余晓玲 LIN Zhi-Mei;LI Jian;RAO Jin;ZOU Zhong-Qing;YU Xiao-Ling(Department of Hematology,Affiliated Hospital of Chengdu University,Chengdu 610081,China)

机构地区：[1]成都大学附属医院血液科,成都610081 [2]成都大学临床医学院,成都610106

出　　处：《中国免疫学杂志》2020年第4期445-450,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(31500657)

摘　　要：目的:探讨KLF4在弥漫性大B细胞淋巴瘤组织中的表达及其对弥漫性大B细胞淋巴瘤细胞增殖、侵袭、迁移能力的影响及作用机制。方法:选择弥漫性大B细胞淋巴瘤石蜡组织标本60例和淋巴结反应增生患者的淋巴结石蜡组织标本60例,免疫组化法测定组织中KLF4表达。Western blot和RT-PCR测定弥漫性大B细胞淋巴瘤细胞系ly1细胞和人B淋巴母细胞系HMy2.CIR细胞中KLF4蛋白、mRNA水平。ly1细胞分为空白对照组(不转染病毒)、阴性对照组(转染阴性对照病毒)和过表达KLF4组(转染过表达KLF4病毒),Western blot和RT-PCR测定ly1细胞中KLF4蛋白、mRNA水平,CCK-8测定转染后ly1细胞增殖情况,Transwell小室法测定转染后ly1细胞侵袭和迁移能力,Western blot法测定转染后三组ly1细胞Akt、p-Akt、PI3K、p-PI3K蛋白水平。结果:弥漫性大B细胞淋巴瘤组织B细胞中KLF4阳性表达率(30.0%)低于对照组织B细胞(100.0%)(P<0.05)。ly1细胞中KLF4蛋白和mRNA水平低于HMy2.CIR细胞(P<0.05)。与空白对照组和阴性对照组比较,过表达KLF4组ly1细胞中KLF4蛋白和mRNA水平升高(P<0.05),A值降低(P<0.05),侵袭和迁移细胞数降低(P<0.05),p-Akt、p-PI3K蛋白水平降低(P<0.05),空白对照组和阴性对照组ly1细胞中各指标比较差异无统计学意义(P>0.05)。结论:弥漫性大B细胞淋巴瘤组织中KLF4水平降低,过表达KLF4可能通过PI3K/Akt信号通路抑制弥漫性大B细胞淋巴瘤细胞增殖和侵袭迁移。Objective:To investigate the expression of KLF4 in diffuse large B-cell lymphoma and its effect on the proliferation,invasion and migration of diffuse large B-cell lymphoma cells and its mechanism.Methods:60 specimens of diffuse large B-cell lymphoma paraffin-embedded and 60 specimens of patients with lymph node reactive hyperplasia were selected.The expression of KLF4 in tissues were determined by immunohistochemistry.The levels of KLF4 protein and mRNA in diffuse large B cell lymphoma cell line ly1 cells and human B lymphoblastoid cell line HMy2.CIR cells were determined by Western blot and RT-PCR.Ly1 cells were divided into blank control group(no transfected virus),negative control group(transfected negative control virus)and overexpressed KLF4 group(transfected overexpressing KLF4 virus).Western blot and RT-PCR were used to determine the level of KLF4 protein and mRNA in ly1 cells.CCK-8 was used to determine the proliferation of ly1 cells after transfection.Transwell chamber assay was used to determine the ability of invade and migrate of ly1 cells after transfection.Western blot was used to determine the Akt,p-Akt,PI3K and p-PI3K protein levels in the three groups of ly1 cells after transfection.Results:The positive expression rate of KLF4 in B cells of diffuse large B-cell lymphoma tissues(30.0%)was lower than that in B cells of control tissues(100.0%)(P<0.05).The level of KLF4 protein and mRNA in ly1 cells were lower than those in HMy2.CIR cells(P<0.05).Compared with the blank control group and the negative control group,the level of KLF4 protein and mRNA were increased(P<0.05),the A value was decreased(P<0.05),the number of invasion and migration cells were decreased(P<0.05),the p-Akt and p-PI3K protein level was decreased(P<0.05)in the overexpressed KLF4 group,there were no significant difference in the indexes between the blank control group and the negative control group(P>0.05).Conclusion:The KLF4 level is decreased in diffuse large B-cell lymphoma tissues,and overexpression of KLF4 may inhibit prolif

关 键 词：人锌指转录因子Krüppel样因子4 弥漫性大B细胞淋巴瘤 磷脂酰肌醇激酶 蛋白激酶B 增殖 侵袭 迁移 

分 类 号：R733.4[医药卫生—肿瘤]