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作 者:王爽[1] 程玉祥[1] 夏德安[1] WANG Shuang;CHENG Yu-Xiang;XIA De-An(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040)
出 处:《植物研究》2020年第1期79-84,共6页Bulletin of Botanical Research
基 金:国家自然科学基金(31570580)~~
摘 要:AtGDPD-Like3是编码甘油磷酸二酯磷酸二酯酶(GDPD)类似基因,拟南芥该家族基因AtGDPD-Like3突变体shv3存在严重的根毛发育缺陷。为了鉴定AtGDPD-Like3关键氨基酸位点,我们构建S 538 A、V 556 A和D 628 A单点突变AtGDPD-Like3,分别转化atgdpdl3突变体并观察其恢复根毛缺陷程度。结果显示V 556 A、D 628 A位点突变AtGDPD-Like3完全恢复atgdpdl3根毛生长缺陷表型,但S 538 A突变AtGDPD-Like3只是部分恢复根毛缺陷。这些结果表明Ser 538是AtGDPD-Like3较为关键的氨基酸位点,突变影响其蛋白质功能行使,同时暗示AtGDPD-Like3还存在其它的关键氨基酸位点。此研究结果为进一步探究AtGDPD-Like3蛋白功能行使的作用机制奠定了基础。AtGDPD-Like3 encode glycerophosphodiester phosphodiesterase-like(GDPD)proteins and shv3,its family gene AtGDPD-Like3 mutant,has serious root hair defects.To identify key amino acid sites in AtGDPD-Like3,we constructed S 538 A,V 556 A and D 628 A single site mutation of AtGDPD-Like3,which were expressed in atgdpdl3 mutants,respectively.The results showed that AtGDPD-Like3 with V 556 A or D 628 A mutation could completely restore root hairs growth defects of atgdpdl3,whereas,AtGDPD-Like3 with S 538 A mutation partially restored root hairs growth defects.These indicate that Ser 538 is the key amino acid site in AtGDPD-Like3,which affects function of its protein.Meanwhile,it implies other key amino acid sites in AtGDPD-Like3.The results of this study would lay a foundation for further exploring the functional mechanism of AtGDPD-Like3 protein.
关 键 词:拟南芥 AtGDPD-Like3 关键氨基酸位点 点突变
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