磁共振T1 mapping、T2 mapping评估大鼠肝纤维化和肝脂肪变性  

Evaluation on rats liver fibrosis and steatosis with MR T1 mapping and T2 mapping

作　　者：汪苍 张香梅[2] 王俊萍[3] WANG Cang;ZHANG Xiangmei;WANG Junping(Department of Radiology,Shenzhen Bao'an Traditional Chinese Medicine Hospital Group,Shenzhen 518133,China;Department of Pathology,the Third People's Hospital of Shenzhen,Shenzhen 518100,China;Department of Gastroenterology,Peking University Shenzhen Hospital,Shenzhen 518036,China)

机构地区：[1]深圳市宝安中医院(集团)影像科,广东深圳518133 [2]深圳市第三人民医院病理科,广东深圳518100 [3]北京大学深圳医院消化内科,广东深圳518036

出　　处：《中国医学影像技术》2020年第2期210-214,共5页Chinese Journal of Medical Imaging Technology

摘　　要：目的采用磁共振T1 mapping、T2 mapping评估大鼠肝纤维化和肝脂肪变性,观察其应用价值。方法将80只大鼠随机分成实验组(n=70)对照组(n=10),分别于背部注射四氯化碳橄榄油溶液及生理盐水,制作大鼠肝纤维化模型。于注药后第4、6、8、10和12周,分别随机选取实验组14只和对照组2只大鼠采集MRI,测量肝实质T1值和T2值,并行组织病理检查。根据病理结果将大鼠肝纤维化划分为S0~S4期,脂肪变性划分为F0~F4度,比较肝纤维化各期T1值和T2值,分析其与肝纤维化及肝脂肪变性相关性。结果 S0期大鼠肝脏T1值和T2值与肝纤维化各期差异有统计学意义(P均<0.05),S1期[(402.01±57.14)ms]肝实质T1值较S3期[(514.83±87.10) ms]和S4期[(518.72±36.50) ms]短(P均<0.05),S2期[(417.49±47.00) ms]肝实质T1值较S4期短(P<0.05);S1期[(65.12±9.46) ms]肝实质T2值较S4期[(55.33±7.30) ms]略延长(P<0.05)。T1值与肝纤维化程度呈正相关(r=0.68,P<0.01),T2值与脂肪变性程度呈正相关(r=0.72,P<0.01)。结论磁共振T1 mapping可无创评估大鼠肝纤维化,T2 mapping可无创评估大鼠肝脂肪变性,有望为临床诊断肝纤维化和肝脂肪变性提供新的影像学方法。Objective To evaluate liver fibrosis and steatosis in rats with MR T1 mapping and T2 mapping, and to explore their application value. Methods Totally 80 rats were randomly divided into experimental group(n=70) and control group(n=10).Rats in experimental group were injected carbon tetrachloride olive oil in the back to build liver rat fibrosis models, while in control group were injected equally amount of saline in the back. MR T1 mapping and T2 mapping were performed on 14 rats of experimental group and 2 rats of control group 4, 6, 8, 10 and 12 weeks after injections. Liver parenchyma T1 value and T2 value were measured, and pathology inspection was performed. The rats with hepatic fibrosis were divided into S0-S4 stages, while with steatosis were divided into F0-F4 degrees according to pathology results. T1 value and T2 value of rat liver of each stage of hepatic fibrosis were compared, and their correlations with liver fibrosis and liver steatosis were analyzed. Results There were statistically differences of liver T1 and T2 values of fibrosis S0 stage rats and each stage of hepatic fibrosis rats(all P<0.05). Liver T1 value of liver fibrosis S1 stage rats [(402.01±57.14)ms] were shorter than that of liver fibrosis S3([514.83±87.10] ms) and liver fibrosis S4([518.72±36.50] ms) rats(both P<0.05). Liver T1 value of liver fibrosis S2 rats([417.49±47.00]ms) was shorter than that of liver fibrosis S4 rats(P<0.05). Liver T2 value of liver fibrosis S1 rats([65.12±9.46]ms) was slightly shorter than that of liver fibrosis S4 rats([55.33±7.30] ms)(P<0.05). There was positive relationship of liver T1 value and liver fibrosis(r=0.68, P<0.01), also of liver T2 value and liver steatosis(r=0.72, P<0.01). Conclusion MR T1 mapping can noninvasively evaluate rat liver fibrosis, while T2 mapping can noninvasively evaluate rat liver steatosis, which are expected to provide new imaging methods for clinical diagnosis of liver fibrosis and liver steatosis.

关 键 词：肝疾病 大鼠 磁共振成像 

分 类 号：R575.5[医药卫生—消化系统] R445.2[医药卫生—内科学]