姜黄素减轻小鼠深部组织压力性损伤  

Curcumin alleviates deep tissue pressure injury in mice

作　　者：刘盼盼 张子锐 杨旭[1] 韩晶[1] 陆连芳[2] 王爱敏[1] 张菊 LIU Pan-pan;ZHANG Zi-rui;YANG Xu;HAN Jing;LU Lian-fang;WANG Ai-min;ZHANG Ju(School of Nursing,Qingdao University,Qingdao 266000;Department of Hepatobiliary Surgery,the Affiliated Hospital of Qingdao University,Qingdao 266000,China)

机构地区：[1]青岛大学护理学院,山东青岛266000 [2]青岛大学附属医院肝胆外科,山东青岛266000

出　　处：《基础医学与临床》2020年第3期299-304,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金青年基金(81701838);中国博士后科学基金(2018M632628)

摘　　要：目的评价姜黄素(Cur)对缺血-再灌注所致小鼠深部组织压力性损伤(DTPI)创面愈合的影响。方法将小鼠随机分为对照组(Con)、模型组(Mod)、姜黄素4和8 mg/kg干预组(Cur 4,Cur 8),每组10只。磁铁压迫法构建深部组织损伤模型,每隔48 h于创面注射姜黄素,拍照观察伤口愈合情况。HE染色观察伤口愈合病理改变,realtime PCR检测肿瘤坏死因子(TNF-α)、白介素-6(IL-6)、白介素-10(IL-10)、血管内皮生长因子(VEGF-α)、转化生长因子-β(TGF-β)的mRNA表达;Western blot检测VEGF-α、TGF-β、Stat3、p-Stat3蛋白的表达。结果与对照组相比,模型组伤口逐渐恶化,病理学改变明显。与模型组相比,Cur 8组伤口愈合明显加快(P<0.05);Cur 8组炎性细胞浸润减少,新生血管密度增多。Cur 8组TNF-α、IL-6的mRNA表达量均低于模型组,IL-10、VEGF-α和TGF-β表达量均高于模型组(P<0.05);Cur 8组VEGF-α、TGF-β、p-Stat3蛋白表达水平均高于模型组(P<0.05)。结论姜黄素可减轻小鼠缺血-再灌注所致的DTPI,其机制可能与抑制炎性反应及促进血管再生有关。Objective To evaluate the effect of curcumin on wound healing in deep tissue pressure injury(DTPI)induced by ischemia-reperfusion in mice.Methods Mice were randomly divided into control group(Con),model group(Mod),4 and 8 mg/kg curcumin treatment group(Cur 4,Cur 8),10 mice in each group.A deep tissue pressure injury model was established by magnet compression.The wounds tissue was injected with curcumin every48 hours and photographed to observe the wound healing.HE staining was used to observe the pathological changes of wound healing.Real-time PCR was used to detect the mRNA expression of TNF-α,IL-6,IL-10,VEGF-αand TGF-β.Western blot was used to detect the expression of VEGF-α,TGF-β,Stat3 and p-Stat3.Results Compared with the control group,the wound in the model group gradually deteriorated,and the pathological changes were obvious.Compared with the model group,the wound healing of the Cur 8 group was significantly accelerated(P<0.05).Compared with the model group,the inflammatory cell infiltration was reduced and the neovascular density was increased in the Cur 8 group.The mRNA expression of TNF-αand IL-6 in the wound tissues of the Cur 8 group was less than those in the model group,while the expression of IL-10,VEGF-αand TGF-βwas higher than those in the model group(P<0.05).The expression of VEGF-α,TGF-βand p-Stat3 in Cur 8 group was higher than those in model group(P<0.05).Conclusions Curcumin administration can alleviate DTPI of mice induced by ischemia-reperfusion,and its mechanism may be related to the inhibition of inflammatory response and promotion of angiogenesis.

关 键 词：姜黄素 深部组织压力性损伤 创面愈合 动物实验 

分 类 号：R471[医药卫生—护理学]