脂多糖修饰对革兰阴性菌毒力及外膜囊泡生物学特性影响的研究进展  

Advance in Effects of LPS Modification on Virulence of G^- Bacteria and Biological Characteristics of OMVs

作　　者：杨君 李冰 蔡汝健 宋帅 勾红潮 楚品品 卞志标 王令 闫鹤[2] 李春玲 YANG Jun;LI Bing;CAI Ru-jian;SONG Shuai;GOU Hong-chao;CHU Pin-pin;BIAN Zhi-biao;WANG Ling;YAN He;LI Chun-ling(Institute of Animal Heath,Guangdong Academy of Agricultural Sciences,Guangdong Provincial Key Laboratory ofLivestock Disease Prevention,Scientific Observation and Experiment Station of Veterinary Medicine,Guangzhou,Guangdong,510640,China;School of Food Sciences and Engineering,South China University of Technology,Guangzhou,Guangdong,510640,China;Maoming City Animal Disease Prevention and Control Center,Maoming,Guangdong,525000,China;Drugs and Diagnostic Techniques of Guangdong Province,Ministry of Agriculture,P.R.China,Guangzhou Animal Epidemic Prevention Supervision Institute,Guangzhou,Guangdong,510640,China)

机构地区：[1]广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室/农业部兽用药物与诊断技术广东科学观测实验站,广东广州510640 [2]华南理工大学食品科学与工程学院,广东广州510640 [3]茂名市动物疫病预防控制中心,广东茂名525000 [4]广州市动物防疫监督所,广东广州510640

出　　处：《动物医学进展》2020年第2期98-103,共6页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31772776,31502107);广东省自然科学基金项目(2015A030313562);广东省科技计划项目(2014A010107020);广州市科技计划项目(201607010380);广东省现代农业产业技术体系创新团队项目(2019KJ119)

摘　　要：脂多糖(LPS)是革兰阴性(G^-)菌的内毒素,刺激机体引发炎症反应,为细菌的一种毒力因子,主要存在于细菌细胞壁外膜上,也是外膜囊泡(OMVs)的主要成份。OMVs是细菌从膜上脱落下来的一种囊状结构,含有大量的LPS、外膜蛋白及其他成分,具有良好的免疫原性,被认为是最具潜力的亚单位疫苗。大多数LPS由类脂A、核心寡糖和O抗原多糖组成,这3个组分的合成影响着LPS的结构,继而影响细菌的毒力和细菌分泌OMVs的产量、毒性及免疫原性等生物学特性。论文主要对LPS的合成转运过程中结构修饰对G^-菌毒力及其OMVs生物学特性的影响进行综述,为革兰阴性菌致病机理研究及开发新型亚单位疫苗提供新思路。Lipopolysaccharides are endotoxins of G^-bacteria that stimulate the body to trigger an inflammatory reaction.They are virulence factors of bacteria,mainly found in the bacterial cell membranes,and are also the main ingredients of OMVs.OMVs are the cystic structure from the cell membrane.They contain a large amount of LPS,outer membrane proteins and other components,and have good immunogenicity.They were considered as the most promising subunit vaccines.Most LPS consist of lipid A,core oligosaccharide and O-antigenic polysaccharides,which affect the structure of LPS and so affect the virulence of bacteria,the production,toxicity and immunogenicity of OMVs secreted by bacteria.This paper reviewed the research progress of the effects of structural modification on the virulence of G^-bacteria and the biological characteristics of OMVs during the synthesis and transport of LPS,to provide new ideas for the research on the pathogenic mechanism of G^-bacteria and the development of new subunit vaccines.

关 键 词：脂多糖 结构 革兰阴性菌 毒力 外膜囊泡 生物学特性 

分 类 号：S852.612[农业科学—基础兽医学] S852.165[农业科学—兽医学]