MicroRNA-613靶向Frizzled7对裸鼠人前列腺癌细胞移植瘤增殖和侵袭的影响  

Effects of microRNA-613 targeting Frizzled7 on proliferation and invasion of human prostate cancer cell xenografts in nude mice

作　　者：段万里[1] 王莉[1] 任伟[1] 邓骞 梁亮[1] 孙羿[1] 程永毅[1] DUAN Wan-li;WANG Li;REN Wei;DENG Qian;LIANG Liang;SUN Yi;CHENG Yong-yi(Department of Urology,Shaanxi Provincial People s Hospital,Xi'an 710061,China)

机构地区：[1]陕西省人民医院泌尿外科

出　　处：《现代泌尿外科杂志》2020年第2期174-179,共6页Journal of Modern Urology

基　　金：陕西省重点研发计划——一般项目-社会发展领域（No.2018SF-040）

摘　　要：目的观察microRNA-613(miR-613)对裸鼠人前列腺癌细胞移植瘤生长及侵袭的影响。方法在4组裸鼠腋下皮下分别注射对数生长期的前列腺癌细胞PC3-miR-NC、DU145-miR-NC和稳定表达miR-613的PC3-miR-613、DU145-miR-613细胞,建立裸鼠移植瘤模型。采用免疫组化检测FZD7的表达,采用Western blot检测FZD7、β-catenin、p-β-catenin、E-cadherin、Vimentin蛋白的表达。结果PC3-miR-613、DU145-miR-613组移植瘤体积分别小于PC3-miR-NC、DU145-miR-NC组(P<0.05),且FZD7、β-catenin、p-β-catenin、Vimentin蛋白相对表达量降低,E-cadherin相对表达量增高。结论miR-613靶向FZD7抑制裸鼠人前列腺癌细胞移植瘤生长和侵袭进程。Objective To observe the effects of microRNA-613(miR-613)on the growth and invasion of human prostate cancer cell xenografts in nude mice.Methods The prostate cancer cells PC3-miR-NC,DU145-miR-NC,PC3-miR-613 and DU145-miR-613 were subcutaneously injected into nude mice to establish xenograft models.The expression of FZD7 was detected with immunohistochemistry,and the protein expressions of FZD7,β-catenin,p-β-catenin,E-cadherin and Vimentin were detected with Western blot.Results Compared with PC3-miR-NC and DU145-miR-NC groups,PC3-miR-613 and DU145-miR-613 groups had smaller tumor volumes,lower relative expressions of FZD7,β-catenin,p-β-catenin and Vimentin,but higher expression of E-cadherin(P<0.05).Conclusion The miR-613 targeting FZD7 inhibits the growth and invasion of human prostate cancer cell xenografts in nude mice.

关 键 词：前列腺癌 microRNA-613 FZD7 增殖 侵袭 移植瘤 

分 类 号：R737.25[医药卫生—肿瘤]