海茸中L-岩藻糖及硫酸基的测定  

Determination of L-Fucose and Sulfate in Seaweed

作　　者：颜丽 刘秀河[1] 赵方铭 李同乐 YAN Li;LIU Xiu-he;ZHAO Fang-ming;LI Tong-le(Qilu University of Technology(Shandong Academy of Sciences),Ji'nan 250353,China;Ji'nan Brown Algae Bioengineering Co.,Ltd.,Ji'nan 250353,China)

机构地区：[1]齐鲁工业大学(山东省科学院),济南250353 [2]济南褐藻生物工程有限公司,济南250353

出　　处：《中国调味品》2020年第2期137-141,共5页China Condiment

基　　金：十三五国家重点研发计划(2017YFB038600);国家级大学生创新创业训练计划项目(201710431046)

摘　　要：采用比色法测定L-岩藻糖,L-岩藻糖的吸光值在0~18μg/mL呈良好的线性关系;采用热水浸提法提取海茸中L-岩藻糖,其含量为5.790%,回收率为94.46%,重复性和精密度良好。采用硫酸钡比浊法测定海茸中L-岩藻糖中硫酸基含量,硫酸根浓度的吸光值在0~24μg/mL范围内线性关系良好,测定SO42-含量为3.746%,回收率为95.28%,相对标准偏差为3.68%,重复性和精密度良好。最后测定海茸中褐藻胶含量为74.5%。L-fucose is determined by colorimetry,and the absorbance value of L-fucose has a good linear relationship from 0μg/mL to 18μg/mL,L-fucose is extracted by hot water extraction,the content is 5.790%,the recovery rate is 94.46%,the repeatability and precision are good.Barium sulfate turbidimetric method is used to determine the content of sulfuric acid in L-fucose of seaweed.The absorbance value of sulfuric acid has a good linear relationship in the range of 0~24μg/mL.The content of SO42- is 3.746%,the recovery rate is 95.28%,the relative standard deviation is 3.68%,and the repeatability and precision are good.Finally,the content of sodium alginate in seaweed is 74.5%.

关 键 词：海茸 L-岩藻糖 硫酸基含量 比色法 硫酸钡比浊法 

分 类 号：TS201.1[轻工技术与工程—食品科学]