钙激活中性蛋白酶2在口腔鳞状细胞癌中的表达及对肿瘤细胞生物学行为的影响  

Calpain 2 expression in oral squamous cell carcinoma and its effect on tumor cells

作　　者：郑博 廖湘凌[1] 丁南[1] 王敬国 庞晓霞 Bo Zheng;Xiang-ling Liao;Nan Ding;Jing-guo Wang;Xiao-xia Pang(Department of Stomatology,Beijing Luhe Hospital Capital Medical University,Beijing 101100)

机构地区：[1]首都医科大学附属北京潞河医院口腔科

出　　处：《中国现代医学杂志》2020年第3期27-31,共5页China Journal of Modern Medicine

摘　　要：目的探究钙激活中性蛋白酶2(Calpain-2)在口腔鳞状细胞癌(OSCC)中的表达及Calpain-2对肿瘤细胞生物学行为的影响。方法通过RT-PCR、Western blotting和免疫组织化学方法检测OSCC组织Calpain-2的表达,分析Calpain-2与临床病理的关系。利用敲低Calpain-2的OSCC细胞评价Calpain-2对细胞增殖、凋亡及迁移的影响。结果OSCC组织中Calpain-2表达水平升高(P<0.05)。Calpain-2敲低的OSCC细胞增殖能力和凋亡率与对照组比较,差异无统计学意义(P>0.05)。敲低Calpain-2的表达能抑制OSCC细胞的迁移能力(P<0.05)。结论Calpain-2可能参与肿瘤细胞的迁移过程,有可能成为抑制肿瘤细胞迁移的重要靶点。Objective To investigate the expression of calcium-activated neutral protease Calpain-2 in oral squamous cell carcinoma(OSCC)and the effect of Calpain 2 on tumor cells.Methods The expression of Calpain-2 in OSCC tissues was detected by RT-PCR,Western blotting and immunohistochemistry,and the relationship between Calpain-2 and clinicopathology was analyzed.OSCC cells without Calpain-2 were used to evaluate the effects of Calpain-2 on cell proliferation,apoptosis and migration.Results The expression of Calpain-2 was increased in OSCC clinical samples(P<0.05).There was no significant difference in proliferation and apoptotic rate of OSCC cells without Calpain-2 compared with the control group(P>0.05).Knocking down the expression of Calpain-2 significantly inhibited the migration of OSCC cells(P<0.05).Conclusions Calpain-2 may be involved in the migration of cancer cells and may become an important target for inhibiting the migration of cancer cells.

关 键 词：口腔鳞状细胞癌 钙激活中性蛋白酶2 迁移 

分 类 号：R739.85[医药卫生—肿瘤]