溃疡性结肠炎患者血清miR-15表达水平与炎性反应状态及预后的关系  

Relationship between the expression of serum miR-15 and inflammatory status and prognosis in patients with ulcerative colitis

作　　者：刘丽[1] 柳云恩 LIU Li;LIU Yunen(Department of Internal Medicine,the Third Hospital Affiliated to Liaoning University of Traditional Chinese Medicine,Shenyang 110004,China;Department of Emergency Medicine,the General Hospital of Northern Theater Command,Shenyang 110016,China)

机构地区：[1]辽宁中医药大学附属第三医院内科,辽宁沈阳110004 [2]北部战区总医院急诊医学部,辽宁沈阳110016

出　　处：《国际消化病杂志》2020年第1期41-46,共6页International Journal of Digestive Diseases

基　　金：辽宁省自然科学基金资助项目(2013021098)

摘　　要：目的探讨溃疡性结肠炎(UC)患者血清miR-15的表达水平与炎性反应状态及预后的关系。方法选取2014年1月至2017年1月辽宁中医药大学附属第三医院及北部战区总医院收治的101例UC患者作为研究对象,其中UC缓解期45例,UC活动期56例,另选取同期50例健康体检者作为对照组。通过RT-qPCR法检测所有受试者血清miR-15的相对表达量,比较不同程度UC患者血清miR-15的表达水平。分析血清miR-15表达水平与Mayo评分、Baron评分的相关性。采用多因素Logistic回归法分析影响UC患者复发的相关因素。结果UC活动期患者血清miR-15的相对表达量显著高于UC缓解期和对照组,差异均有统计学意义(P均<0.05)。UC活动期重度组患者血清miR-15的相对表达量、Mayo评分、Baron评分显著高于中度组和轻度组患者,差异均有统计学意义(P均<0.05)。相关性分析显示,miR-15的相对表达量与Mayo评分、Baron评分均呈正相关(P均<0.05)。多因素Logistic回归分析显示,性别、吸烟、pANCA、基底浆细胞增多、miR-15相对表达量与UC患者的复发密切相关。结论UC患者血清miR-15的相对表达量与炎性反应状态呈正相关,且与UC患者的复发关系密切。Objective This paper attempts to investigate the relationship between the expression of serum miR-15 and inflammatory status and prognosis in patients with ulcerative colitis(UC).Methods One hundred and one UC patients admitted to the Third Hospital Affiliated to Liaoning University of Traditional Chinese Medicine and the General Hospital of Northern Theater Command from January 2014 to January 2017 were selected as the subjects,including 45 UC patients in remission stage and 56 UC patients in active stage.Fifty healthy persons in the same period were assigned as the control group.The relative expression of serum miR-15 of the three groups was detected by RT-qPCR,and the relative expression of serum miR-15 was compared.The correlation between the relative expression of serum miR-15 and the Mayo score and the Baron score was analyzed.Besides,the multivariate logistic regression was used to analyze the related factors affecting the recurrence of UC patients.Results The relative expression of serum miR-15 in patients with active UC was significantly higher than that in patients with remission of UC and the control group(all P<0.05).The relative expression of serum miR-15,the Mayo score,and the Baron score in severe UC patients in the active phase were significantly higher than those in moderate and mild UC patients(P<0.05).The correlation analysis showed that the relative expression of miR-15 was positively correlated to the Mayo score and the Baron score(P<0.05).The multivariate logistic regression analysis showed that sex,smoking,pANCA,basal plasma cell proliferation,and miR-15 were closely related to recurrence in UC patients.Conclusion The relative expression of serum miR-15 in UC patients is positively correlated to the inflammatory state,and is closely related to the recurrence of UC patients.

关 键 词：溃疡性结肠炎 miR-15 炎性反应状态 预后 

分 类 号：R574.62[医药卫生—消化系统]