缺钙下枳根抗氧化系统及细胞壁果胶的变化  

Changes of antioxidant system and cell wall pectin in Poncirus trifoliate L. under calcium deficiency

作　　者：刘亚林 闫磊 曾钰 姜存仓[1] LIU Yalin;YAN Lei;ZENG Yu;JIANG Cuncang(Microelement Research Center/College of Resources and Environment,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学微量元素研究中心/华中农业大学资源与环境学院

出　　处：《华中农业大学学报》2020年第1期61-66,共6页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目（41271320）;中央高校基本科研业务费专项（2017PY055）

摘　　要：选取枳实生苗为试验材料,设置正常钙(1.23 mmol/L)和缺钙(0 mmol/L)2个处理进行水培试验。结果显示,缺钙处理会使枳根生物量和细胞壁的提取率下降,根超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)的活性分别下降31.96%、71.27%、35.91%、45.70%。并且,缺钙导致砧木总钙、细胞壁钙、螯合态果胶钙和碱溶态果胶钙含量显著降低。其中,根系细胞壁螯合态果胶和碱溶态果胶含量分别下降35.16%、40.51%。通过X射线衍射技术(XRD)分析发现,缺钙对根系细胞壁纤维素的结晶度影响不大。因此,缺钙条件下根系抗氧化酶系统活性的降低以及细胞壁果胶含量的大幅度下降,造成了细胞壁丧失抵御钙胁迫的能力,导致细胞壁不能维持其正常功能,进而严重阻碍了枳根系的生长。Poncirus trifoliate L.was used to be treated with normal calcium(1.23 mmol/L)and deficient calcium(0 mmol/L)levels.The hydroponics results showed that deficient calcium treatment reduced the root biomass and the extraction rate of root cell wall.The activities of antioxidant enzymes(SOD,CAT,POD,APX)decreased by 31.96%,71.27%,35.91%and 45.70%,respectively.Calcium deficiency significantly decreased total calcium,cell wall calcium,chelated pectin calcium and alkali-soluble pectin calcium in root.Among which,the content of chelated pectin and alkali-soluble pectin in root cell wall decreased by 35.16%and 40.51%,respectively.Results of XRD analyses showed that calcium deficiency had little effect on the crystallinity of cellulose in root cell wall.Under deficient calcium condition,the activities of antioxidant enzymes and the content of pectin in root cell wall decreased dramatically,resulting in the loss of the ability of cell wall to resist calcium deprivation and the failure of cell wall to maintain its normal function,thus seriously hindering the growth of trifoliate rootstock root.

关 键 词：枳 砧木根系 抗氧化酶系统 细胞壁 果胶 钙胁迫 酶活性 

分 类 号：S606[农业科学—园艺学] S666