斑马鱼白血病模型的研究进展  

Research Progress of Zebrafish Leukemia Model

作　　者：向文碧 何志旭[1,2,4] 舒莉萍 XIANG Wenbi;HE Zhixu;SHU Liping(National&Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique/Center for Tissue Engineering and Stem Cell Research,Guizhou Medical University,Guiyang 550004,China;Key Laboratory of Adult Stem Cell Translational Research,Chinese Academy of Medical Sciences,Guiyang 550004,China;Department of Pediatrics,Guizhou Medical University,Guiyang 550004,China;Department of Pediatrics,the Affiliated Hospital of Zunyi Medical University,Zunyi 563004,China)

机构地区：[1]贵州医科大学细胞工程生物医药技术国家地方联合工程实验室组织工程与干细胞实验中心,贵阳550004 [2]中国医学科学院成体干细胞转化研究重点实验室,贵阳550004 [3]贵州医科大学儿科学教研室,贵阳550004 [4]遵义医科大学附属医院儿科学教研室,贵州遵义563004

出　　处：《医学综述》2020年第2期263-268,共6页Medical Recapitulate

基　　金：中国医学科学院中央级公益性科研院所基本科研业务费项目(2017PT31042);贵州省研究生导师工作室建设项目(黔教研合GZS〔2016〕02);贵州省科技计划项目(黔科合平台人才〔2017〕5611);贵阳市科技计划项目(筑科合同〔2017〕5-18)

摘　　要：斑马鱼是一种脊椎动物有机体,因具有保守的造血过程和独特的实验优势,尤其适合用于研究人类白血病。目前,研究斑马鱼的最新技术包括转基因技术、基因组编辑技术和移植技术,通过这些技术现已构建了许多白血病模型。斑马鱼的通体透明与先进的谱系追踪和成像技术相结合,揭示了白血病发生、发展的许多细节。凭借这些优势,斑马鱼成为白血病研究中具有独特优势的模式生物之一,此外,基于斑马鱼的高通量药物筛选的进步有望加速新型白血病疗法的开发。The zebrafish is a vertebrate organism with a conserved hematopoietic program and unique experimental strengths suiting it for the investigation of human leukemia.Recent technological advances in zebrafish research including efficient transgenesis,precise genome editing,and straightforward transplantation techniques have led to the generation of a number of leukemia models.The transparency of the zebrafish when coupled with improved lineage-tracing and imaging techniques has revealed exquisite details of leukemic initiation and progression.With these advantages,the zebrafish represents a unique experimental system for leukemic research and additionally,advances in zebrafish-based high-throughput drug screening promise to hasten the development of novel leukemia therapeutics.

关 键 词：白血病 斑马鱼 动物模型 

分 类 号：R733.7[医药卫生—肿瘤]