七株硫酸盐还原菌汞甲基化能力的比较  

Methylation of mercury in seven sulfate-reducing bacteria

作　　者：职亭亭 吴浩贤 郭瑛瑛 毛宇翔[1] 何滨[2] 阴永光[2] 史建波[2] 胡立刚[2] 江桂斌[2] ZHI Tingting;WU Haoxian;GUO Yingying;MAO Yuxiang;HE Bin;YIN Yongguang;SHI Jianbo;HU Ligang;JIANG Guibin(School of Resource and Environment,Henan Polytechnic University,Jiaozuo,454003,China;Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,State Key Laboratory of Environmental Chemistry and Ecotoxicology,Beijing,100085,China)

机构地区：[1]河南理工大学资源环境学院,焦作454003 [2]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085

出　　处：《环境化学》2019年第12期2657-2664,共8页Environmental Chemistry

基　　金：国家自然科学基金(21777179)资助~~

摘　　要：目前硫酸盐还原菌汞甲基化的研究集中在Desulfovibrio desulfuricans ND132菌株上,由于D.desulfuricans ND132并非商业化提供的菌株,使得国内很多实验室难以获得该菌株开展微生物汞甲基化的相关研究.本文收集了7株商业化的硫酸盐还原菌,通过系统研究探寻其中可用作替代的汞甲基化模式菌株.在实验室培养条件下,通过对甲基化关键基因(hgcA和hgcB)、生长特性、汞的耐受性以及汞甲基化能力的测试,结果发现,Desulfomicrobium escambiense(CGMCC 1.3481)生长较快、具有较好的汞甲基化率(7.5%±0.7%),具备作为汞甲基化模式菌株的潜力.Desulfovibrio desulfuricans ND132 is most widely used strain for studies on mercury methylation of sulfate-reducing bacteria(SRB).It however is not a commercially available strain,limiting the relevant studies in most of domestic laboratories.In this study,seven commercially available strains of sulfate-reducing bacteria were collected and explored as the potential model for methylation of mercury with SRB.The occurrence of key gene for mercury methylation(hgcA and hgcB),growth characteristics,tolerance to mercury and mercury methylation ability,were systematically evaluated.The results showed that Desulfomicrobium escambiense(CGMCC 1.3481)had a conversion rate of up to 7.5%±0.7%under the optimized condition,which could serve as a model strain in studies on mercuric methylation with SRB.

关 键 词：汞 甲基化 硫酸盐还原菌 甲基汞 模式菌株 

分 类 号：X172[环境科学与工程—环境科学]