长尾管蚜蝇Eristalis tenax(Linnaeus,1758)线粒体基因组测序及分析  

Sequencing and Analysis of the Mitochondrial Genome of Eristalis tenax(Linnaeus,1758)

作　　者：张玉波[1,2] 史娅琴 孙翠英 Zhang Yubo;Shi Yaqin;Sun Cuiying(Anshun University,Anshun,561000;Key Laboratory of Insect Informative System and Resource Development and Utilization of Guizhou Province,Anshun,561000)

机构地区：[1]安顺学院,安顺561000 [2]贵州省昆虫信息系统与资源开发利用重点实验室,安顺561000

出　　处：《基因组学与应用生物学》2019年第12期5371-5378,共8页Genomics and Applied Biology

基　　金：贵州省教育厅黔教合人才团队(编号:201571)、贵州省教育厅重点项目(编号:黔教合KY字2014271);贵州省科技厅三方联合基金(编号:黔科合LH字20157689);安顺学院博士基金项目(asubsjj201702)共同资助

摘　　要：为了研究长尾管蚜蝇在双翅目的系统发育地位,本研究采用全基因组鸟枪法获得其线粒体基因组95%左右的序列,长度为15996 bp,登录号为MH159199,包含13个蛋白编码基因,2个rRNA基因,22个tRNA基因,共有9处基因重叠区和16处基因间隔区。13个蛋白编码基因的起始密码子均为ATN,终止密码子除ND5为T外其余均为TAA。从线粒体基因组水平探讨了双翅目3亚目12科28种的系统进化地位,结果表明:食蚜蝇科和潜蝇科聚在一起形成无缝组与以丽蝇科、麻蝇科、寄蝇科、蝇科、花蝇科等构成的有缝组形成姊妹群。In order to explore the phylogenetic position of Eristalis tenax, about 95% of the mitochondrial genome of Eristalis tenax was sequenced using shotgun method. GenBank accession number is MH159199. The sequence length was 15 996 bp including 13 protein coding genes, 22 tRNA genes and 2 rRNA gene, 9 gene overlap regions and 16 intergenic region. All protein coding genes used ATN as initiation codon. All protein coding genes used TAA or TAG as stop codon except ND5 that used incomplete T as stop codon. The phylogenetic analysis was made from the level of mitochondria genome based on 23 species of 12 families, which belong to 3 suborder of the Diptera. The result indicated that that Aschiza including Syrphidae and Agromyzidae was sister group to Scllizophora including 7 families: Calliphoridae, Sarcophagidae, Muscidae, Tachinidae,Anthomyiidae, Tephritidae, Sepsidae.

关 键 词：长尾管蚜蝇 密码子偏好性 线粒体基因组 系统发育 

分 类 号：Q963[生物学—昆虫学]