p53蛋白和MDR表型在白血病中的共表达研究  

Coexpression of p53 Protein and MDR Phenotypes in Leukemia

作　　者：李焕杰[1] Li Huanjie(Hospital of Shandong Institute of Business and Technology University,Yantai,264005)

机构地区：[1]山东工商学院医学院

出　　处：《基因组学与应用生物学》2019年第12期5664-5670,共7页Genomics and Applied Biology

摘　　要：白血病的最重要的特征性耐药机制之一,是由P-糖蛋白(Pgp)和多药耐药相关蛋白(MRP)介导的多药耐药性(MDR)。除了Pgp和MRP之外,p53突变或失活可能在治疗失败中起一定作用。一些研究已经证明Pgp和MRP可能与突变或失活的p53蛋白的过表达相关联。本研究的目的是通过流式细胞术(FCM)分析p53表达与MDR功能表型之间的关联。采用流式细胞仪分析罗丹明123检测MDR功能表型。发现41例慢性粒细胞白血病(CML)中有18例(43.9%)为阳性,28例慢性淋巴细胞白血病(CLL)中有16例(54.1)为阳性,28例急性髓性白血病(AML)中有11例(39.3%)为阳性,22例急性淋巴细胞白血病(ALL)中有4例(18.2%)为阳性。在白血病细胞中观察到不同水平的p53表达:41例CML中有12例(29.2%),28例CLL中有9例(32.1%);28例AML中有15例(53.6%);22例ALL中有8例(36.4%)。本次研究中,在ALL、CLL和AML中未观察到p53表达与MDR功能表型之间的有显著关联。而在CML中观察到p53与MDR功能表型的共表达有显著关联(p=0.000 3)。在该疾病的加速期和,p53过表达更频繁地出现。研究结果表明MDR功能表型可能与白血病晚期p53突变有关。One of the most important characteristic resistance mechanisms of leukemia is multidrug resistance(MDR) mediated by P-glycoprotein(Pgp) and multidrug resistance-associated protein(MRP). In addition to Pgp and MRP, mutations or inactivation of p53 may play a role in treatment failure. Some studies have demonstrated that Pgp and MRP may be associated with overexpression of mutated or inactivated p53 protein. The purpose of this study was to analyze the association between p53 expression and the functional phenotype of MDR by flow cytometry(FCM). Flow cytometry was used to analyze rhodamine 123 for detection of MDR function phenotypes.41 out of 41 chronic myelogenous leukemia(CML) were found to be positive, 16(54.1%) of 28 chronic lymphocytic leukemia(CLL) were positive, and 28 were acute myeloid leukemia(AML) Of 11 patients(39.3%) were positive,4(18.2%) of the 22 acute lymphoblastic leukemia(ALL) were positive. Different levels of p53 expression were observed in leukemic cells: 12(29.2%) of the 41 CML cases;9(32.1%) of the 28 CLL cases;and 15(53.6%) of the 28 AML cases. There were 8(36.4%) cases in 22 cases of ALL. Conclusions: In this study, no significant association between p53 expression and MDR function phenotype was observed in ALL, CLL and AML. A significant association between coexpression of p53 and MDR functional phenotype was observed in CML(p=0.000 3). In the accelerated phase of the disease, p53 overexpression occurs more frequently. The results of the study suggest that the functional phenotype of MDR may be related to the late leukemic p53 mutation.

关 键 词：慢性粒细胞白血病 急性骨髓性白血病 多药耐药性 P53蛋白 罗丹明123 流式细胞术 

分 类 号：R733.7[医药卫生—肿瘤]