EAMG大鼠动物模型肾上腺免疫相关性研究  

Study on the correlation of adrenal immunity in EAMG rat model

作　　者：王静 方丽洁 刘颖[3] 姜雄 况时祥 WANG Jing;FANG Lijie;LIU Ying(Department of Integrated Traditional Chinese and Western Medicine,Wuhan Wudong Hospital,Wuhan 430084,China)

机构地区：[1]武汉市武东医院中西医结合科,湖北武汉430084 [2]武汉市武东医院药剂科,湖北武汉430084 [3]湖北中医药大学临床医学院,湖北武汉430061 [4]遵义市中医院神经内科,贵州遵义563000 [5]贵州中医药大学第二附属医院神经内科,贵州贵阳550003

出　　处：《中风与神经疾病杂志》2020年第2期123-128,共6页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No.81360605)。

摘　　要：目的探讨EAMG大鼠肾上腺的免疫相关性,及强的松的治疗机制,以阐明EAMG的发病机制。方法36只Lewis大鼠随机分为模型组、对照组、强的松组3组,每组12只。观察各组EAMG大鼠Lennon评分,并通过ELISA法检测EAMG大鼠血清AchR-Ab含量水平,通过PCR法检测肾上腺中IFN-γmRNA、TGF-βmRNA、TNF-αmRNA、IL-2 mRNA、IL-4 mRNA、IL-17 mRNA的表达。结果与对照组比较,模型组大鼠肾上腺中IFN-γmRNA、TGF-βmRNA、IL-4 mRNA表达减少,TNF-αmRNA、IL-17 mRNA的表达增加,且有显著差异;与模型组比较,强的松组大鼠肾上腺中IFN-γmRNA表达减少,TGF-βmRNA、IL-4 mRNA、TNF-αmRNA、IL-4 mRNA、IL-17 mRNA的表达增加;EAMG大鼠肾上腺中没有IL-2 mRNA的表达。结论强的松能有效改善EAMG大鼠Lennon评分,降低EAMG大鼠血清AchR-Ab水平,保护EAMG大鼠肾上腺细胞TGF-βmRNA、IL-4 mRNA的表达,减少肾上腺细胞TNF-αmRNA、IL-17 mRNA、IFN-γmRNA的表达,这可能是其治疗MG的作用机制之一。Objective To investigate the correlation of adrenal glands in EAMG rats and the treatment mechanism of prednisone,to elucidate the pathogenesis of EAMG.Methods Thirty-six lewis rats were randomly divided into model group,control group and prednisone group,with 12 rats in each group.Observe Lennon score of EAMG rats in each group,and detect serum AChR-Ab level of EAMG rats by ELISA.Detect expression of IFN-γ mRNA、TGF-β mRNA、TNF-mRNA、IL-2 mRNA、IL-4 mRNA、IL-17 mRNA in adrenal gland by PCR.Results Compared with the control group,the expressions of IFN-γ mRNA、TGF-β mRNA、IL-4 mRNA in the adrenal glands of the model group were decreased,while the expressions of TNF-α mRNA、IL-17 mRNA were increased in significant differences.Compared with the model group,the expression of IFN-γ mRNA in the adrenal glands of the prednisone group decreased,while the expression of TGF-β mRNA、IL-4 mRNA、TNF-α mRNA、IL-4 mRNA、IL-17 mRNA increased.There was no expression of IL-2 mRNA in the adrenal glands of EAMG rats.Conclusion Prednisone can effectively improve the Lennon score of EAMG rats,reduce the serum AChR-Ab level of EAMG rats,protect the expression of TGF-β mRNA、IL-4 mRNA in adrenal cells of EAMG rats,and reduce the expression of TNF-α mRNA、IL-17 mRNA、IFN-γ mRNA in adrenal cells of EAMG rats,which may be one of the mechanisms of its treatment for MG.

关 键 词：实验性重症肌无力 肾上腺 免疫 

分 类 号：R746.1[医药卫生—神经病学与精神病学]