新型心肌灌注显像药物99^Tc^m-3SPboroxime的SPECT显像实验研究  

Experimental study of SPECT imaging with novel myocardial perfusion imaging tracer: ^99Tc^m-3SPboroxime

作　　者：席笑迎 张宗耀[1] 张利霞[1] 韩凯 赵祚全 方纬[1] Xi Xiaoying;Zhang Zongyao;Zhang Lixia;Han Kai;Zhao Zuoquan;Fang Wei(Department of Nuclear Medicine,Fuwai Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100037,China)

机构地区：[1]北京协和医学院、中国医学科学院阜外医院核医学科,100037

出　　处：《中华核医学与分子影像杂志》2020年第2期69-74,共6页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：国家自然科学基金(81771872)。

摘　　要：目的在健康及急性心肌梗死小型猪模型中验证^99Tc^m-3SPboroxime(3SP)作为快速心肌灌注显像(MPI)药物的优势。方法制备^99Tc^m-Teboroxime(TEBO)和^99Tc^m-3SP。取2只健康小型猪,麻醉后经静脉分别注射370 MBq ^99Tc^m-TEBO或^99Tc^m-3SP,立即用标准SPECT动态采集平面图像,持续采集20 min,测量心脏、肝和肺的放射性计数及心/肝、心/肺比值,并观察其随时间的变化;取4只健康小型猪和1只急性心肌梗死模型猪,麻醉后经静脉分别注射370 MBq ^99Tc^m-TEBO和^99Tc^m-3SP,同1只猪体内2种显像剂的注射分别在连续2 d内完成,同时用碲锌镉心脏专用SPECT仪(CZT-SPECT)动态采集断层图像15 min,测量心肌、肝的放射性计数及心/肝比值,评价MPI图像质量。采用配对t检验分析数据。结果^99Tc^m-TEBO和^99Tc^m-3SP的放化纯均>95%。^99Tc^m-3SP的初始心肌摄取与^99Tc^m-TEBO接近[平面显像,注射后2 min:309.32×103与314.13×103计数/MBq;断层显像,注射后2 min(已校正):7.96±0.87与8.24±1.53,t=0.277,P>0.05],但心肌滞留优于^99Tc^m-TEBO[平面显像,注射后20 min:218.67×103与143.19×103计数/MBq;断层显像,注射后15 min(已校正):6.76±0.45与5.06±0.33,t=-12.412,P=0.001]。两者肝摄取及心/肝比值相似(注射后15 min内,t值:-1.332~1.101,均P>0.05)。CZT-SPECT MPI显示,正常心肌对^99Tc^m-3SP呈分布均匀的高摄取,梗死心肌呈放射性缺损改变。在^99Tc^m-3SP注射后15 min内的任意5 min显像时间窗中,正常与心肌梗死小型猪均可获取高质量的SPECT图像。结论^99Tc^m-3SP心肌摄取较高,注射后20 min内均有明显的心肌滞留,SPECT图像质量优越,是一种生物学性能优异的^99Tc^m标记快速MPI药物。Objective To assess the superiority of ^99Tc^m-3SPboroxime(^99Tc^m-3SP for short)as a fast-myocardial perfusion imaging(MPI)tracer in normal and acute myocardial infarction(AMI)mini-swine.Methods ^99Tc^m-3SP and ^99Tc^m-Teboroxime(^99Tc^m-TEBO for short)were prepared.Approximately 370 MBq ^99Tc^m-3SP or ^99Tc^m-TEBO was injected intravenously in 2 healthy mini-swine separately.Dynamic planar images were acquired immediately after injection and continued for 20 min using a standard SPECT camera.The radioactivity uptakes in the heart,liver,and lungs were measured,and heart/liver and heart/lung ratios over time were calculated.Dynamic SPECT studies were performed in 4 normal swine and 1 AMI-swine using cadmium zinc telluride-SPECT(CZT-SPECT).List mode acquisitions were immediately started and continued for 15 min after intravenous injection of approximately 370 MBq ^99Tc^m-TEBO and ^99Tc^m-3SP.The injection of two radiotracers in the same swine was completed within 2 d.The radioactivity uptakes in heart and liver were measured,and heart/liver ratio was calculated.Image quality was also evaluated.Paired t test was used to analyze the data.Results The radiochemical purity of ^99Tc^m-TEBO or ^99Tc^m-3SP were both above 95%.The initial heart uptake of ^99Tc^m-3SP was very close to that of ^99Tc^m-TEBO(planar image,2 min postinjection:309.32×103vs 314.13×103 counts/MBq;SPECT image,2 min postinjection(corrected):7.96±0.87 vs 8.24±1.53,t=0.277,P>0.05),but the myocardial retention time was much longer than that of ^99Tc^m-TEBO(planar image,20 min postinjection:218.67×103vs 143.19×103 counts/MBq;SPECT image,15 min postinjection(corrected):6.76±0.45 vs 5.06±0.33,t=-12.412,P=0.001).The uptake of liver and heart/liver ratio between ^99Tc^m-TEBO and ^99Tc^m-3SP were similar(t values:-1.332-1.101,all P>0.05 within 15 min).SPECT MPI images demonstrated uniform tracer distribution with clearly visualizable myocardial boundary in normal myocardium and intense perfusion defect in infarct myocardium.High quality SPECT images c

关 键 词：心肌灌注显像 肟类 硼酸化物 锝 体层摄影术 发射型计算机 单光子 猪 

分 类 号：R[医药卫生]