预靶向策略在抗体放射性标记中的研究进展  

Research progress of pretargeting strategies in antibody radiolabeling

作　　者：周慧敏 朱小华 Zhou Huimin;Zhu Xiaohua(Department of Nuclear Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院核医学科,武汉430030

出　　处：《中华核医学与分子影像杂志》2020年第2期118-121,共4页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：国家自然科学基金(81873903)。

摘　　要：与传统放射性核素体外标记抗体靶向显像不同,预靶向策略利用特定亲和偶联系统,将肿瘤特异性抗体与放射性核素分别修饰,先后注射入体内,利用亲和偶联系统间的高度特异性及亲和性在体内将放射性核素定位于抗体所在靶点,实现放射性标记,达到显像的目的。该法可减少放射性核素的血液循环时间,从而减少正常组织的放射性分布,促进短半衰期核素的应用。该文就目前最主要的4种预靶向策略:生物素-链霉亲和素(SA)系统、双特异性抗体(BsAb)系统、互补寡核苷酸序列和生物正交点击化学反应体系进行综述。Pretargeting is an imaging strategy different from the imaging of traditional radiolabeled antibody,in which the antibody and radioisotope are administered in turn and then combined by coupling system within the body.Due to the high specificity and affinity between the coupling systems,the radioisotope is localized to the target in vivo,and then the radioactive labeling is achieved for imaging.Nuclear imaging using pretargeting methods can decrease the circulation duration of the radioisotope so as to reduce the uptake of the radioactivity in normal tissues,and facilitate the use of short-lived radioisotopes.This article provides a bird′s-eye view of 4 main strategies:biotin-streptavidin(SA)system,bispecific antibodies(BsAb)system,complementary oligonucleotides sequence,and bioorthogonal click chemistry system.

关 键 词：放射免疫显像 同位素标记 抗体 发展趋势 

分 类 号：R73[医药卫生—肿瘤]