定量斑点印迹分析技术在高通量样品蛋白表达水平检测中的应用  

Application of quantitative dot blot analysis technology for protein expression level detection

作　　者：刘琳 门婷婷[2] 张文凤 张建棣 米佳 杨春华 LIU Lin;MEN Tingting;ZHANG Wenfeng;ZHANG Jiandi;MI Jia;YANG Chunhua(Department of Pharmacy,Binzhou Medical University,Yantai 264003 Shandong,P.R.China;Department of Nursing,Binzhou Medical University;Yantai Zestern Biotechnique Co.LTD;Medicine and Pharmacy Research Center,Binzhou Medical University)

机构地区：[1]滨州医学院药学院,山东烟台264003 [2]滨州医学院护理学院 [3]烟台载通生物技术有限公司 [4]滨州医学院医药研究中心

出　　处：《滨州医学院学报》2020年第1期11-14,22,共5页Journal of Binzhou Medical University

基　　金：山东省重点研发项目(2018GSF118131)

摘　　要：目的检测定量斑点印迹(QDB analysis)技术在高通量组织样品和细胞样品蛋白表达水平定量检测中的应用情况。方法采用QDB analysis技术检测87只小鼠的前列腺组织中tubulin的相对表达水平,以及8种人细胞系中tubulin的绝对表达水平(即蛋白真正表达水平)。结果通过QDB analysis技术发现小鼠前列腺组织中tubulin蛋白的表达水平根据小鼠周龄不同而有所不同;8种人细胞系中tubulin蛋白的绝对表达水平在90~140 fmol/μg之间。结论QDB技术可适用于高通量组织样品和细胞样品中蛋白表达水平的相对定量和绝对定量检测。Objective To detect the application of quantitative dot blot analysis(QDB analysis)detection technology for protein expression level analysis by determining the protein level of high throughput tissue samples and cell samples.Methods The relative expression of tubulin in the prostate tissues from 87 mice and the absolute expression of tubulin from 8 human cell lines were detected by QDB analysis.Results The results showed that the expression level of tubulin protein in prostate tissue from mice was different according to the age,and the absolute expression level of tubulin protein in 8 cell lines was between 90~140 fmol/μg.Conclusion QDB analysis can be applied to the relative and absolute quantification in a high throughput tissue samples and cell samples.

关 键 词：蛋白质表达 定量检测 定量斑点印迹技术 蛋白质免疫印迹技术 TUBULIN 

分 类 号：Q-331[生物学]