分子印迹电化学传感器检测水产品中孔雀石绿  

Determination of malachite green in aquatic products by electrochemical sensor based on molecularly imprinted polymer

作　　者：陈宏[1,3] 靖玉 孙思阳 王悦 朱玉 励建荣 CHEN Hong;JING Yu;SUN Si-yang;WANG Yue;ZHU Yu;LI Jian-rong(College of Chemistry and Chemical Engineering,Bohai University,Jinzhou 121013,China;College of Food Science and Technology,Bohai University,Jinzhou 121013,China;National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Jinzhou 121013,China)

机构地区：[1]渤海大学化学化工学院,辽宁锦州121013 [2]渤海大学食品科学与工程学院,辽宁锦州121013 [3]生鲜农产品贮藏加工及质量安全控制技术国家地方联合工程技术研究中心,辽宁锦州121013

出　　处：《化学研究与应用》2020年第2期208-213,共6页Chemical Research and Application

基　　金：“十二五”国家科技支撑计划(2012BAD29B06)资助;泰山学者蓝色产业领军人才团队支撑计划项目(鲁政办字(2015)19号)资助;中央引导地方科技发展专项资金项目(2018107005)资助

摘　　要：以孔雀石绿(MG)为模板,以邻氨基酚为功能单体通过循环伏安法(CV)在玻碳电极表面上聚合制备了MG分子印迹电化学传感器,用CV法和差分脉冲法(DPV)研究了传感器的响应性能,并用于水产品中MG检测。结果表明,DPV峰电流减少量与MG浓度在0.02~0.5 μg·mL^-1范围内呈线性关系,线性方程为ΔI(μA)=26.77CMG+2.663;在0.5~2.5 μg·mL^-1范围内呈线性关系,线性方程为ΔI(μA)=2.662CMG+14.25,检出限为1.2×10-2μg·mL^-1。MG分子印迹电化学传感器操作简单快捷,具有较好的特异性、重复性和稳定性,可满足水产品中MG的快速检测需要。An electrochemical sensor has been developed for the selective determination of malachite green(MG)using the molecularly imprinted technique.A molecular imprinted polymer(MIP)film on the surface of a glassy carbon electrode(GCE)was prepared by electropolymerization of o-aminophenol(OAP)in the presence of MG in the sodium perchlorate(NaClO4)solution using cyclic voltammetry(CV).The electrochemical performance of the sensor was studied using CV and differential pulse voltammetry(DPV).The results showed that the peak current difference increased with the increase of MG concentration in the range 0.02~0.5 μg·mL^-1 with a calibration equation of ΔIpc (μA)=26.77CMG+2.663 and in the range 0.5~2.5μg·mL^-1 with a calibration equation of ΔIpc (μA)=2.662CMG+14.25.The detection limit was estimated as 0.012μg·mL^-1(S/N=3).The MIP-POAP/GCE exhibited excellent reproducibility,good stability and good selectivity.The results suggest that the proposed MIP-POAP/GCE could be used to determine the concentration of MG in aquatic samples.

关 键 词：电化学传感器 分子印迹 孔雀石绿 水产品 

分 类 号：O657.1[理学—分析化学]