氨肽酶P2在结直肠癌组织中的表达及其与临床病理特征和预后的关系  

Expression of XPNPEP2 in colorectal cancer tissues and its association with clinicopathological features and prognosis

作　　者：曾瑶 徐惠丽 王梦漪 张子龙 ZENG Yao;XU Huili;WANG Mengyi;ZHANG Zilong(Department of Oncology,The Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,China)

机构地区：[1]华中科技大学同济医学院附属武汉中心医院肿瘤科,武汉430014 [2]华中科技大学同济医学院附属荆州医院,荆州434020

出　　处：《实用医学杂志》2020年第2期210-215,共6页The Journal of Practical Medicine

基　　金：湖北省自然科学基金面上项目(编号：2019CFB809)

摘　　要：目的探讨氨肽酶P2(XPNPEP2)在结直肠癌组织中的表达及其与患者临床病理特征和预后的关系。方法采用免疫组织化学方法检测113例配对的结直肠癌组织和癌旁组织中XPNPEP2的表达情况,采用卡方检验分析XPNPEP2表达水平与患者临床病理特征的关系,Kaplan⁃Meier法绘制不同XPNPEP2表达水平结直肠癌患者无复发生存和总体生存曲线,组间生存差异采用Log⁃rank检验进行比较,并进一步通过单因素和多因素Cox比例风险回归模型分析影响结直肠癌预后的独立危险因素。结果结直肠癌组织中XPNPEP2蛋白的表达水平显著高于癌旁组织(P<0.001),且XPNPEP2高表达与肿瘤浸润深度、脉管浸润及TNM分期显著相关(均P<0.05),而与患者性别、年龄、肿瘤位置、肿瘤大小、分化程度及淋巴结转差异移均无统计学意义(均P>0.05)。XPNPEP2高表达组结直肠癌患者的无复发生存率和总生存率均显著低于低表达组(均P<0.05)。COX多因素分析示XPNPEP2表达水平是影响患者无复发生存率和总生存率的独立危险因素(均P<0.05)。结论XPNPEP2蛋白在结直肠癌中表达上调,其高表达与多种不良临床病理特征及预后密切相关,可作为判断结直肠癌患者预后的重要生物标志物。Objective To investigate the expression of XPNPEP2 in colorectal cancer(CRC)tissues and its association with clinicopathological characteristics and prognosis of CRC patients.Methods Immunohistochem⁃istry method was used to detect the expression of XPNPEP2 in 119 CRC tissues and matched tumor⁃adjacent tissues,and chi⁃square test was used to analyze the relationship between XPNPEP2 expression and clinicopathological features of CRC.The recurrence⁃free survival and overall survival curves were drawn by using the Kaplan⁃Meier method,and the difference between survival curves were compared by Log⁃rank test.Furthermore,univariate and multivariate Cox proportional hazards regression models were adopted to assess the independent risk factors that affecting the prognosis of CRC.Results The expression of XPNPEP2 in CRC tissues was significantly higher than that in para⁃cancerous tissues(P<0.001),and it was correlated with tumor invasion depth,vascular invasion and TNM stage(all P<0.05),but not with patient′s gender,age,tumor location,tumor size,degree of differentia⁃tion and lymph node metastasis(all P>0.05).The recurrence⁃free survival rate and overall survival rate of patients with high expression of XPNPEP2 were significantly lower than those in the low⁃expression group(both P<0.01).Further Cox regression analysis showed that XPNPEP2 expression was an independent prognostic factor that affecting CRC patients′RFS(HR:1.66,95%CI:1.19~2.96,P=0.015)and OS(HR:5.01,95%CI:1.49~16.82,P=0.009).Conclusion XPNPEP2 was up⁃regulated in CRCand its expression was closely related with several unfavorable clinicopathological features and prognosis of CRC.These results indicated that XPNPEP2 might be an important biomarker for predicting the prognosis of CRC.

关 键 词：XPNPEP2 结直肠癌 免疫组化 临床病理特征 预后 

分 类 号：R735.3[医药卫生—肿瘤]